长江中下游地区亚洲镰刀菌NX-2毒素群体的检测

Detection of NX-2 producing Fusarium asiaticum strains in the Middle and Lower Reaches of Yangtze River

由禾谷镰刀菌复合种(Fusarium graminearum species complex,FGSC)引起的麦类赤霉病,是农业生产上的重要病害。为明确中国长江中下游冬小麦主产区小麦赤霉病菌种的构成及其地理分布,对2008年从江苏、浙江和湖北3省采集的656株小麦赤霉病菌株进行了分类鉴定。结果显示,其中558个菌株为亚洲镰刀菌(Fusarium asiaticum),98个菌株为禾谷镰刀菌(Fusarium graminearum sensu stricto),表明中国长江中下游冬小麦主产区小麦赤霉病的主要致病菌是亚洲镰刀菌。选择亚洲镰刀菌(F.asiaticum)为研究对象,通过PCR-RFLP的方法对其进行产NX-2毒素菌株的检测。结果没有检测到产NX-2毒素菌株,表明中国长江中下游冬小麦主产区并未出现NX-2毒素群体。本研究旨在了解NX-2毒素群体在中国长江中下游地区的地理分布,为进一步研究麦类赤霉病菌群体遗传多样性和演化趋势奠定基础,为麦类赤霉病的防治和毒素污染的控制提供理论依据。

Fusarium head blight（FHB）caused by Fusarium graminearum species complex is a very important disease in agricultural production.To analyze the composition and geographic distribution of F.graminearumspecies complex in the main areas of wheat production in the Middle and Lower Reaches of Yangtze River in China,wheat spikes with FHB symptoms were collected from-3 provinces including Jiangsu,Zhejiang and Hubei in2008.A total of 656 strains were isolated.The results showed that 558 strains were F.asiaticum and 98 strains were F.graminearum s.str among the 656 isolates.Population of F.asiaticum is predominant.F.asiaticum was selected to detect NX-2 producing strains through the PCR-RFLP method.The results showed that there were no NX-2 producing strains in the main areas of wheat production in the Middle and Lower Reaches of Yangtze River in China.The aim of this research is to get a detailed geographic distribution of NX-2 producing strains in the Middle and Lower Reaches of Yangtze River in China,lay the foundation for further study of the population genetic diversity,and provide theoretical basis for disease and mycotoxin control.