甘草黄酮纳米乳的制备及其稳定性研究

Preparation of Glycyrrhiza Flavonoids Nano Emulsion and Evaluation of Its Stability

[目的]通过绘制伪三元相图优选处方的方法,制备了甘草黄酮纳米乳并对其稳定性进行评价。[方法]以EL-40、Span-80为表面活性剂,肉豆蔻酸异丙酯(IPM)为油相,组成混合溶液,以甘油为助表面活性剂制备甘草黄酮纳米乳,将甘草黄酮纳米乳应用高温、高湿、恒温加速试验检验其稳定性。[结果]纳米乳处方为:0.2 g甘草黄酮、1.2 g EL-40、0.3 g Span-80、0.5 g甘油、0.5 g IPM、4.0 g蒸馏水,经试验验证甘草黄酮纳米乳仍为澄清透明纳米乳状液,未出现分层现象,性质稳定。[结论]该方法操作简单,纳米乳稳定性良好。

[Objective] To prepare Glycyrrhiza flavonoids nano emulsion by drawing the pseudo ternary phase diagram optimization prescription and to evaluate its stability. [Methods] The EL-40 and Span-80, isopropyl myristate（IPM）, and glycerol were used as surfactant, oil phase, and cosurfactant respectively. They were mixed to prepare Glycyrrhiza flavonoids nano emulsion. The accelerated test in the conditions of high temperature, high humidity and constant temperature was conducted respectively to evaluate the stability of the Glycyrrhiza flavonoids nano emulsion. [Results] The determined prescription was as following： 0.2 g of Glycyrrhiza flavonoids, 1.2 g of EL-40, 0.3 g of Span-80, 0.5 g of glycerol, 0.5 g IPM, and 4.0 g distilled water.The prepared Glycyrrhiza flavonoids nano emulsion was clear and transparent without layer, and it was evaluated as stable in accelerated test. [Conclusion] The developed technology in this study for preparation of Glycyrrhiza flavonoids nano emulsion is simple to operate and the nano emulsion exhibits good stability.