酵母硒对乌珠穆沁羊免疫球蛋白及细胞因子的影响

Effect of Dietary Supplementation of Selenium-enriched Yeast on Immunoglobulin and Cytokine of Ujumqin Sheep

[目的]通过饲养试验研究日粮中添加酵母硒对乌珠穆沁羊免疫球蛋白及关键细胞因子的影响,为合理确定内蒙古锡林郭勒地区乌珠穆沁羊日粮中酵母硒的适宜添加量以及酵母硒在该地区肉羊养殖中的推广使用提供理论依据。[方法]采用单因子随机试验设计,将240只健康乌珠穆沁羯羊随机分成4组(Ⅰ、Ⅱ、Ⅲ、Ⅳ组)。其中,Ⅰ组为对照组,日粮中添加无机硒(亚硒酸钠)0.3 mg/kg(DM基础),其他3组为酵母硒组,日粮中分别添加0.3、0.6、0.9 mg/kg(DM基础)的酵母硒。预饲期15 d,正饲期60 d。[结果]在日粮中添加酵母硒能够显著提高乌珠穆沁羊血清中免疫球蛋白IgM、IgG、IgA以及细胞因子IL-1、IL-2及IL-6的含量。[结论]在乌珠穆沁羊日粮中添加酵母硒能显著提高乌珠穆沁羊的免疫水平,综合饲养成本及免疫增强效果,以添加0.6 mg/kg(DM基础)效果最佳。

[Objective] In order to rationally determine the optimal supplementation level of Selenium-enriched Yeast in diet of Ujumqin Sheep in Xilingol region of Inner Mongolia and to provide theoretical basis for the promotion and application of Selenium-enriched Yeast in local mutton sheep industry, the effect of dietary supplementation of Selenium-enriched yeast on immunoglobulin and key cytokine of Ujumqin Sheep was evaluated in this study. [Methods] The experiment was conducted by randomized design with a single factor. A total of 240 healthy wether of Ujumqin Sheep were included in this study and were randomly divided into 4 groups（ Ⅰ, Ⅱ, Ⅲ, Ⅳ group）. The group Ⅰ was served as control group, of which the diet was supplemented with 0.3 mg/kg of inorganic selenium（sodium selenite,DM basis）. The Ⅱ, Ⅲ and Ⅳ groups were served as experimental groups, of which the diet were supplemented with 0.3, 0.6, 0.9 mg/kg of Selenium-enriched Yeast（DM basis）. The pre-feeding period lasted for 15 d and the formal-feeding period lasted for 60 d. [Results] The serum content of IgM, IgG, IgA and IL-1, IL-2, IL-6 of Ujumqin Sheep were significantly increased when their diet was supplemented with Selenium-enriched yeast. [Conclusion] The dietary supplementation of Selenium-enriched yeast was able to enhance the immunity function of Ujumqin Sheep. In view of the feeding cost and immunopotentiation effect, supplementation level of 0.6 mg/kg Selenium-enriched yeast（DM basis） in diet was optimal and recommended.