玉米皮固态培养混合益生菌的研究

Solid-state cultivation of mixed probiotics using corn bran

本试验以玉米皮为主要原料,通过单因素试验和正交试验对黑曲霉与酿酒酵母的混合固态培养进行了研究。以蛋白酶、纤维素酶、活菌数为主要考察指标,优化得出的最佳培养条件为:尿素添加量0.5%(P<0.05)、含水量60%、麸皮添加量15%(P<0.05)、黑曲霉接种量20%、培养天数7 d;混菌培养接种比(黑曲霉∶酿酒酵母)为3∶1,混菌共培养1 d。在此条件下,纤维素酶的酶活为622.11 U/g,蛋白酶的酶活为606.55 U/g,每克发酵物含益生活菌总数超过百亿。

Solid-state culture of Aspergillus niger and Saccharomyces cerevisiae was studied by using corn bran as main material.Single-factor and orthogonal experiment were used to optimize the nutritional and environmental conditions for improving the activity of protease,cellulase and the number of living cells.The optimized cultivation conditions for Aspergillus niger were as follows:urea content 0.5%(P < 0.05),water content 60%,wheat bran adding level 15%(P <0.05),inoculation amount 20%,cultivation period 7 days.When co-culture of two strains was conducted,the ratio of Aspergillus niger to Saccharomyces cerevisiae was 3∶1,and co-culture time was 1 day.Under the above conditions,the cellulase activity reached to 622.11U/g,the protease activity achieved 605.55U/g,and the total number of living probiotic cells reached to more than ten billion per gram.