Blf-ADT对HeLa细胞的抑制作用及其机制的研究

Study on Anti-Tumor Activity and Mechanisms of Blf-ADT

研究Blf-ADT(布洛芬硫化氢供体物)的抗肿瘤作用,并对其机制作初步探讨.通过形态学观察、噻唑蓝(MTT)比色实验、集落形成实验测定He La细胞的增殖能力;细胞划痕实验测定He La细胞的迁移能力;Annexin VFITC/PI双染流式细胞仪测定He La细胞的凋亡率;Western blotting检测细胞凋亡相关蛋白(Bcl-2、Bax、Erk、P38和Procaspase-3等)的表达.结果显示,Blf-ADT能呈浓度或时间依赖性抑制细胞增殖、减少细胞集落的形成,降低细胞的迁移能力,同时能显著提高He La细胞的凋亡率,影响细胞凋亡相关蛋白(Bcl-2、Bax、Erk、P38和Procaspase-3等)的表达.Blf-ADT对人宫颈癌He La细胞的生长有显著的抑制作用,对治疗宫颈癌具有潜在的临床应用价值.

The purpose of this study was to observe the activity and mechanisms of a new compound Blf-ADT on He La cells. The effects of Blf-ADT on Hela cells was investigated by morphological observation,MTT assay and colony formation assay,respectively. Wound healing assay was used to detect the effect of Blf-ADT on the migration of He La cells.Meanwhile,the activities of Blf-ADT on apoptosis was measured by Flow cytometry. Furthermore,Western blot was performed to investigate the expressions of related proteins in He La cell line treated with Blf-ADT. As a result,we found that Blf-ADT could inhibit cell proliferation and reduce cell migration in a dose-and time-dependent manner. In addition,Blf-ADT could significantly increase the rate of apoptosis and affect the expression of apoptosis-related proteins.Taken together,the data indicate that Blf-ADT can significantly inhibit the growth of He La cells and have potential clinical value for treatment of cervical cancer.