梅花鹿PRDX4编码区全长cDNA的克隆及序列分析

cDNA Coding Region Cloning and Sequence Analysing of Sika Deer Peroxiredoxin 4

过氧化还原酶4(PRDX4)是重要的抗氧化蛋白,在鹿茸角柄骨膜致敏区特异性高表达。为探究其在鹿茸再生中的作用,本研究克隆了梅花鹿PRDX4的cDNA编码区。序列分析表明梅花鹿PRDX4的cDNA编码区长819 bp,编码272个氨基酸,预测该酶分子量3.062 kD,等电点6.1。利用生物信息学软件预测其是一种跨膜蛋白,有一段信号肽序列和两个保守功能域,并对梅花鹿PRDX4蛋白的三级结构进行了预测。

Peroxiredoxin 4（PRDX4） is an important protein with an antioxidative function,which specifically highly express in sensitization zone of pedicle periosteum of sika deer. To explore the role of PRDX4 in antler regeneration,we cloned the cDNA coding region of sika deer PRDX4 in this study. The sequences analysis revealed that the length of cDNA coding region of sika deer PRDX4 was 819 bp. The DNA sequence encoded a protein of 272 amino acids. The calculated molecular weight and isoelectric point of the enzyme were 3.062 kD and 6.1,respectively. We predicted that it was a transmembrane protein with a signal peptide and two conserved functional domains,and we predicted the tertiary structure of sika deer PRDX4.