下司犬FGF5基因mRNA的表达与绒毛性状的相关性分析

Analysis of Relevance between FGF5 Gene m RNA Expression and Fibre Traits in Xiasi Dog

本试验目的在于研究贵州省下司犬长剑与短剑对成纤维生长因子5(FGF5)基因的表达,利用实时荧光定量PCR技术,分别测定长剑下司犬与短剑下司犬的下丘脑、垂体与皮肤组织中FGF5基因的表达水平,并测定耆甲部、体侧部与尾中部毛长。试验结果表明:长剑母犬在皮肤组织中的表达量最高,显著高于垂体、下丘脑等组织的表达(p<0.05),长剑公犬在各组织中的表达差异不显著(p>0.05);短剑母犬在垂体组织中的表达量最高,显著高于下丘脑组织(p<0.05);耆胛部、体侧部、尾中部长剑母犬较长剑公犬毛长,短剑母犬较短剑公犬毛长,但差异都不显著(p>0.05)。该研究为FGF5基因控制毛长性状的机制提供理论基础,对下司犬的研究提供科学依据。

The purpose of the study was to study the expression of long-hair and short-hair of Xiasi dog in Guizhou province on fibroblast growth factor-5 gene. The expression of FGF5 gene in hypothalamic, pituitary and skin tissues of long-hair and short-hair Xiasi dog were determined, and withers, body side parts and central tail length of the hair were measured by SYBR Green Real-time PCR method. The results showed that expression of long-hair female dog was the highest in skin tissues, which was significantly higher than that of pituitary and hypothalamic （p〈0.05）, while expression of male dog was not significant in organization （p〉0.05）; Expression of short-hair female dog was the highest in pituitary tissues, which was significantly higher than in hypothalamic （p〈0.05）; In withers, body side parts and tail in the middle, hair of long-hair female dog was longer than that of male dog, while hair of short-hair female dog was longer than that of male dog, of which differences were not significant （p〉0.05）. This study would provide theoretical basis for controlling mechanism of FGF5 gene in long wool traits, which also provided scientific basis for study of Xiasi dog.