水稻生物钟相关基因的表达特征鉴定及其功能预测分析

Identification of Expression Characteristics and Predictive Analysis of Function of Circadian-related Genes in Rice

水稻作为我国重要农作物,其营养生长和生殖发育过程都受到严格的生物钟的控制,因此,生物钟基因的表达变化也是决定水稻产量和种子质量的主要决定因素之一。为了更全面系统的了解生物钟基因在水稻生长和发育过程中的功能,本研究采用生物信息学共表达方法,筛选和鉴定水稻和拟南芥中生物钟基因,并对比分析了这些生物钟基因在单双子叶模式植物中的可能功能。从水稻表达谱公共数据库中筛选与生物钟基因表达密切相关732个基因,并对筛选出的水稻生物钟相关基因进行表达特性、节律性、生物功能预测及其与拟南芥的对比分析,结果表明水稻和拟南芥的生物钟基因可能都具有在生物钟核心振荡器部分功能相对保守的特点。功能预测分析也还表明水稻生物钟基因参与了8类生物学过程,尤其在应激反应和代谢过程的方面具有明显富集性,从而间接佐证了水稻对于外界环境的响应及其代谢过程具有严格时间调控的分子机理。

Rice is a vital crop in China,and rice vegetative and reproductive process is regulated by the circadian clock. Thus,the expression change of circadian genes could be a determinant in influencing the rice production and quality. To better understand the function of circadian genes in rice vegetative and reproductive process,herein circadian genes from rice and Arabidopsis were screened and the comparison of these genes was executed to analyze the possible function between dicot ant monocot by using bioinformatics tools. About 732 genes were found to be correlated with circadian genes from rice expression profile in public database. Analysis in expression characteristic,circadian rhythm and biological function indicated that circadian genes between rice and Arabidopsis could possess relatively conserved function in the core oscillator of circadian clock. Functional analysis suggested that circadian genes could be mainly involved in eight biological process,especially in stimulus response and metabolic process. These indirect evidences partly explain the molecular mechanism of the timely control of metabolic process and external environment response in rice.