摘要
以灵芝蛋白质的提取率为指标,考察液料比(V/W)、时间、温度及p H值对提取率的影响,在单因素试验的基础上,采用响应面分析方法确定灵芝蛋白质的最优提取工艺。结果表明,灵芝蛋白质最佳提取工艺条件为液料比(V/W)120∶1,温度50℃,p H值10,时间60 min,沉淀p H值3,在此优化工艺下灵芝粗蛋白的实际提取率达39.7%。
The protein extraction rate is taken as index to study the effect of ratio of material：liquid- material ratio（V/W）,extraction time, temperature and p H on extraction yield. On the basis of single factor experiments, the optimized protein extraction process is analyzed by response surface method. The results show that the optimal Ganoderma lucidum protein extraction conditions are as follows： liquid- material ratio（V/W） of 120 ∶1, extraction temperature of 50 ℃, p H of 10,extraction time of 60 min,and precipitation p H of 3. And the real extraction ratio obtained by these optimized conditions is 39.7%.
出处
《农产品加工》
2016年第3期33-36,39,共5页
Farm Products Processing
基金
国家自然科学基金青年基金项目(31501432)
关键词
灵芝蛋白质
响应面分析法
提取工艺
Ganoderma lucidum protein
response surface analysis
extraction process
