茶树新品系“53-34”父本的EST-SSR标记鉴定

Identification of Male Parents for “53-34” Tea Cultivar Based on SSR Markers

本研究采用EST-SSR标记对茶树新品系＂53-34＂及其母本和4个可能父本进行了分析。结果表明：24对引物共检测到69个等位位点,平均2.88;53-34与湘波绿及4个可能父本的相同位点数分别占其扩增总位点数的85.71%、71.43%、65.71%、60.00%、74.26%,最大为湘波绿,最小为福云7号。6份供试材料间相似系数变幅为0.44~0.77,平均0.57。根据相似系数矩阵按UPGMA法进行聚类,在相似系数平均值0.57处可将参试的6份材料划分为三大类：第Ⅰ类高芽齐;第Ⅱ类湘波绿、53-34与古蔺牛皮茶;第Ⅲ类云大72-04与福云7号。参试材料的相似系数和亲缘关系树状图在分子水平上显示了53-34的4个可能父本中,古蔺牛皮茶与其亲缘关系最近,可能为53-34的父本。

EST-SRR primers were used to identify the male parents for 53-34 tea cultivar among 4 possible male parents. The result showed that totally 69 alleles were amplifi ed using 24 core EST-SSR primers, on average of 2.88.The percentage of common alleles with 53-34 is 85.71%, 71.43%, 65.71%, 60.00%, 74.26%, the maximum is Xiangbolv, the minimum is Fuyun 7. The similarity coeffi cient among different cultivars varied from 0.44-0.77, on average of 0.57. The 6 accessions were classifi ed into 3 groups based on the UPGMA method with the similarity coeffi cient at 0.57, and the group Ⅰ contained Gaoyaqi; the group Ⅱ contained Xiangbolv, 53-34 and Gulin-niupicha; the group Ⅲ contained Yunda 72-04, Fuyun 7. The results of the similarity coeffi cient and dendrogram showed a closer relationship between 53-34 and Gulinniupicha, Gulin-niupicha is the possible male parent of 53-34.