^(60)Co辐照对卵清蛋白结构和抗氧化性的影响

Effect of ^(60)Co radiation on structure and antioxidant activity of ovalbumin

采用6种钴60辐照剂量(0、2、4、6、8、10 k Gy)处理鸡蛋卵清蛋白(OVA),并探明辐照技术对卵清蛋白结构和抗氧化性的影响。以新鲜鸡蛋为原料,经0、2、4、6、8、10 k Gy 6种钴60辐照剂量处理;硫酸铵沉淀法提取卵清蛋白;并测定不同辐照条件下卵清蛋白溶解度、自由巯基含量、内源性荧光、还原力、DPPH自由基清除率的变化。实验结果表明:辐照对于卵清蛋白的结构和理化性质影响较大,随着辐照剂量的增加,OVA的可溶性蛋白含量、自由巯基含量、DPPH清除能力和还原力,均有不同程度的提高;内源荧光呈现先上升后下降趋势,当辐照剂量为10 k Gy时,内源荧光值显著低于对照组(p<0.05)。此研究为辐照卵清蛋白产品的工业应用提供了理论依据。

By studying changes on ovalbumin（OVA） from eggs treated by radiation,the effect of radiation on structure and antioxidant activity of OVA were measured. The OVA were treated by 6 radiation doses 0,2,4,6, 8,and 10 k Gy,respectively. Ovalbumin extracted from fresh eggs with the method of ammonium sulfate precipitation.Solubility,free sulfhydryl conten,fluorescence spectroscopy,reducing power and DPPH radical scavenging of the OVA were determined. The results showed that with the increase of radiation intensity,free sulfhydryl contents,protein solubility,reducing power and DPPH increased. However,intrinsic fluorescence firstly increased and then decreased. When the irradiation does increased to 10 k Gy,intrinsic fluorescence was significantly lower than the control group. This study could provide the theoretical basis for radiated ovalbumin industrial applications.