黄参籽可溶性酯结合酚和糖苷结合酚的组成及其抗氧化活性

Phenolic composition and antioxidant activity of soluble ester-bound and glycoside-bound phenolics from Sphallerocarpus gracilis seeds

采用HPLC-MS对黄参籽可溶性酯结合酚和糖苷结合酚的组成进行了分析,并通过DPPH自由基清除活性、FRAP、TEAC、对羟基自由基介导的2-脱氧-D-核糖裂解的抑制作用以及对DNA氧化损伤的保护作用对黄参籽酚提取物的抗氧化能力进行了评价。结果显示,黄参籽可溶性酯结合酚中主要的酚类化合物为咖啡酸、木犀草素葡萄糖苷和儿茶酸葡萄糖苷,可溶性糖苷结合酚中主要的酚类化合物为儿茶酸葡萄糖苷;黄参籽可溶性酯结合酚的DPPH自由基清除活性、铁还原力和Trolox等量抗氧化活性均弱于可溶性糖苷结合酚;相反,对羟基自由基介导的2-脱氧-D-核糖裂解的抑制作用强于可溶性糖苷结合酚;测试浓度范围内,黄参籽酚提取物对DNA氧化损伤具有不同程度的保护作用,且可溶性酯结合酚提取物酚含量与保护作用之间存在显著(p<0.05)的正相关。

The phenolic composition of soluble ester-bound and glycoside-bound phenolics from S. gracilis seeds was analyzed by HPLC-MS. The antioxidant activity of phenolic extracts from S. gracilis seeds was evaluated by DPPH radical scavenging capacity,FRAP,TEAC,inhibitory effects on hydroxyl radical induced2-deoxy-D-ribose degradation and DNA damage protective effect. Results showed that the predominant phenolic compounds were caffeic acid,luteolin-glucoside,and catechin glucoside in soluble ester-bound phenolics. And the major phenolic compound was catechin glucoside in soluble glycoside-bound phenolics.The DPPH radical scavenging capacity,FRAP and TEAC of soluble ester-bound phenolics were lower than those of soluble glycoside- bound phenolics. Conversely, the inhibitory effect on hydroxyl radical induced2-deoxy-D-ribose degradation of soluble ester-bound phenolics was higher than that of soluble glycosidebound phenolics. Significant DNA damage protective effects were observed of soluble ester- bound and glycoside- bound phenolic extracts from S. gracilis seeds, and significant correlation（ p 0. 05） was found between phenolic content of soluble ester-bound phenolics and protective effects.