胰酶酶解珍珠贝分离蛋白制备低苯丙氨酸寡肽制品

Preparation of low-phenylalanine oligopeptide from Pinctada martensii protein isolates by pancreatin

苯丙酮酸尿症(PKU)是一种常见的氨基酸代谢性疾病,低苯丙氨酸饮食对于PKU患者非常重要。本研究以珍珠产业副产物马氏珠母贝肉为原料,提取分离蛋白后,采用胰酶酶解、超滤分级及活性炭吸附分离技术制备低苯丙氨酸寡肽营养功能基料。研究结果显示:随着酶解时间的进行,酶解液中<5 ku的寡肽含量呈现先增加后减少趋势(p<0.05),酶解1 h时,酶解液中所含的寡肽含量最多;经过超滤分级后,>5 ku和<300 u的酶解液中仍存在一些寡肽残留,其中>5 ku组分中寡肽含量为10.43%,<300 u组分含量为24.52%,而300 u^5 ku组分中寡肽含量达到了68.38%;<300 u组分的苯丙氨酸含量最高达到9.52%,活性炭对芳香族氨基酸的吸附量均高于其他氨基酸,300 u^5 ku组分经过活性炭吸附后得到1.29%的低苯丙氨酸寡肽溶液。可以以此为基料开发辅助治疗苯丙酮尿症、吸收率高的低苯丙氨酸寡肽营养功能制品。

Phenylketonuria（PKU） was a common amino acid metabolic diseases,low-phenylalanine diet was important for PKU patients. In this study,the pearl industry byproducts-pearl oyster meat was as raw meat,and the low-phenylalanine oligopeptides were preparation by separation of proteins,pancreatin digestion,ultrafiltration grade and activated carbon adsorption separation. The results showed that with the advance of hydrolysis time,the content of 5 ku oligopeptide first increased and then decreased（p0.05）,the oligopeptide content reached the highest when hydrolysis 1 h. There was still some residual oligopeptide in the 5 ku and 300 u hydrolyzate after ultrafiltration,which 5 ku components oligopeptide content was 10.43%, 300 u component24. 52 %, while 300 u ~ 5 ku component oligopeptide content reached 68. 38 %. Phenylalanine component of300 u component was up to 9.52%,activated carbon adsorption of aromatic amino acids were higher than other amino acids,the phenylalanine component of 300 u^5 ku was reached 1.29% after the activated carbon adsorption. This could be used as the base material to prepare of low phenylalanine oligopeptide nutritional function products for phenylketonuria.