桑叶总黄酮对2型糖尿病模型大鼠肝脏的保护作用

Effect of total flavonoids of mulberry leaves on the liver and mechanisms in type 2 diabetic rats

目的:探讨桑叶总黄酮对2型糖尿病(T2DM)大鼠肝脏的保护作用。方法:选用雄性wistar大鼠,高脂饲料喂养且用链脲佐菌素诱导,复制T2DM模型,模型成功后随即分成模型组、二甲双胍组、桑叶总黄酮高、中、低(200、100、50 mg·kg^(-1)·bw^(-1))剂量组,各药干预4周后,比较各组大鼠的体重和空腹血糖值;称重肝脏,计算肝脏指数;测定血清中甘油三酯(TG)、总胆固醇(TC)和丙氨酸氨基转移酶(ALT)水平;测定肝脏中肝糖原水平,葡萄糖激酶(GK)活性;对肝脏进行HE染色,做病理组织学检查。结果:与模型组相比,桑叶总黄酮高剂量组能降低T2DM大鼠的体重,为(390.79±77.27)g;降低TC、TG和ALT水平,分别为(2.25±0.58)mmol·L^(-1)、(0.92±0.36)mmol·L^(-1)和(69.36±5.58)U·L^(-1);增加肝糖原含量,为(12.22±1.50)mg·g^(-1);提高GK活性,为(10.33±1.30)m IU·min^(-1)·mg protein^(-1);减轻肝脏脂肪变性,改善肝脏病理。结论:桑叶总黄酮在预防和改善T2DM肝损伤方面具有一定的应用开发潜力。

Objective：To observe the effect of total flavonoids of mulberry leaves on liver in type 2 diabetic rats.Methods：The male wistar rats were fed with high fat diet and streptozotocin to induce the type 2 diabetic model and the model rats were divided into five groups randomly：model group,metformin group,total flavonoids of mulberry leaves dose groups（200,100,50 mg·kg^（-1）·bw^（-1）i.g）. After 4 weeks,levels of weight,blood glucose,organ coefficient,total cholesterol（TC）,triglyceride（TG） and alanine aminotransferase（ALT） in serum were observed;hepatic glycogen,hepatic GK activity,and liver samples were observed pathologically with HE staining. Results：Compared with the model group,total flavonoids of mulberry leaves high dose group could reduce the levels of weight（390.79±77.27） g,TC（2.25±0.58） mmol·L^（-1）,TG（0.92±0.36） mmol·L^（-1） and ALT（69.36±5.58） U·L^（-1）,increase the content of hepatic glycogen（12.22±1.50） mg·g^（-1）,improve the activity of GK（ 10. 33 ± 1. 30） m IU·min^（-1）·mg protein^（-1）, obviously alleviate the histopathologic changes including fatty degeneration. Conclusion：It showed that total flavonoids of mulberry leaves had potential to be developed in the aspect of preventing and improving liver damage in type 2 diabetic.