催情促孕中药对黄牛卵巢静止和持久黄体的治疗效果

Effects of Oestrus- and Pregancy Promoting Traditional Chinese Medicine on Ovary Standstill and Persistent Corpus Luteum in Cattle

本试验旨在探讨催情促孕中药方剂对黄牛卵巢静止和持久黄体的治疗效果。选持久黄体黄牛16头,分成对照组（5头）和试验组（11头）;卵巢静止黄牛17头,分成对照组（5头）和试验组（12头）。试验组每天灌服组方药物400 g/次,7 d为一疗程,停药10 d仍未发情者灌服第二疗程,对照组灌服生理盐水。采用统计临床发情率和ELISA方法检测血清孕酮（P4）、促卵泡素（FSH）和促黄体素（LH）浓度来评价治疗效果。结果表明：灌服本方剂对持久黄体牛总有效率为90.00%,对卵巢静止牛总有效率为83.34%,对照组均未见发情;持久黄体牛P4浓度呈由服药前高浓度的先降后升趋势,,10 d时最低（P〈0.05）;FSH由服药前低浓度呈先升后降趋势,7 d时最高（P〈0.05）;LH浓度呈先升后降趋势,10 d最高（P〈0.05）;卵巢静止牛P4浓度从7 d时开始降低,10 d时降至最低（P〈0.05）后持续升至高于服药前水平（P〈0.05）;FSH浓度变化与持久黄体相近,LH浓度在1～5 d呈逐渐升高,7 d时降至服药前浓度,10 d时由急剧升至峰值（P〈0.05）后迅速降至1 d时水平。结果表明,灌服本中药方剂可以显著促进垂体FSH、LH的释放,激活卵巢机能,促使乏情黄牛返情。

To investigate the treatment effect of the Oestrus- and Pregancy-Promoting Traditional Chinese medicine on persistent corpus luteum and ovary standstill cattle. 16 persistent corpus luteum cattles were selected and divided into control group（5 cattles） and treatment group（11 cattles）, selected 17 ovary standstill cattle then divided into control group（5 cattles） and treatment group（12 cattles）. Treatment groups were fed with 400 g of this prescription per day and 7 days as a course of treatment, then the second course of treatment were taken for anestrus cattle after 10 days by the end of the first course, the control groups were taken with saline accordingly. The estrus rate was analyzed and the concentration of serum progesterone（P4）, follicle-stimulating hormone（FSH） and luteinizing hormone（LH） in serum was respectively detected by ELISA method. Results showed that the total effective rate was 90.00% in persistent corpus luteum cattle and 83.34% in ovary standstill cattle, accordingly compared with the natural estrus cattle was zero in control group. In persistent corpus luteum group, the concentration of P4 showed a trend of decreasing firstly from high level and then increasing, and again decreased to the lowest level on d 10 post-feed（ P 〈0.05）. The concentration of FSH showed a trend of increasing firstly from low level and then decreasing, and again increased to the highest level on d 7（P 〈0.05） The concentration of LH showed a trend of increasing firstly from low level and then decreasing, and again increased to the highest level on d 10（ P 〈0.05）. In ovary standstill group, the concentration of P4 decreased from d 7 to d10, and then gradually increased to the peak on d 21（ P〈0.05）. The trend of concentration of FSH was similar to persistent corpus luteum group. The concentration of LH concentration was gradually increased from d 1 to d 5, decreased on d 7（P 〈0.05）, but sharply increased to the peak（P 〈0.05） on d 10 and then rapidly decreased to the level of d 1. Results showed that feeding with this prescription significantly activated ovarian function and prompted postpartum anestrus cattle to return to estrus via promoting the release of FSH and LH from pituitary and regulating the function of corpus luteum.