响应面法优化乌骨藤通光藤苷G提取工艺

Optimization of Extraction Process for Tenacissima G from Marsdenia by Response Surface Methodology

利用响应面法对乌骨藤Marsdenia tenacissima通光藤苷G的提取工艺进行优化。以通光藤苷G提取率为指标,在单因素试验的基础上,选取乙醇浓度、料液比、提取时间、提取次数进行4因素3水平的Box-Behnken中心组合研究,并运用Design Expect 8.0软件对试验参数进行分析,研究各自变量及其交互作用对通光藤苷G提取率的影响。结果显示,通光藤苷G的最佳提取条件为:乙醇溶液浓度80%,料液比1∶20(W/V),提取时间1.5 h,提取2次,在此条件下,通光藤苷G提取率为0.253%。

The optimum extraction conditions of tenacissima G from Marsdenia tenacissima were investigated. On the basis of single factor test, the alcohol concentration, solid-liquid ratio, extraction duration and extraction times were selected for the four-factor, three-level Box-Behnken experimental design. In addition, Design Expect 8.0 software was employed to analyze the test data and the response surface methodology was applied to optimize the extraction conditions. The results suggested that optimum extraction conditions consisted of 80% ethanol as the extraction solvent, an extraction duration of 1.5 h, a solid-liquid ratio of 1∶20（W/V） and extraction of two times. Under these optimum conditions, the extraction yields of tenacissima G from M. tenacissima was 0.253%.