美洲一枝黄花中天然产物二咖啡酰奎尼酸和绿原酸的分离纯化研究

The Isolation and Purification of Dicaffeoylquinic Acids and Chlorogenic Acid in Solidago Canadensis

研究了入侵植物美洲一枝黄花中的成分,分析鉴定了其中重要成分二咖啡酰奎尼酸和绿原酸的含量并探讨了其分离纯化.美洲一枝黄花不同部位中以花和叶的二咖啡酰奎尼酸和绿原酸有效成分含量最高,其花蕾中的二咖啡酰奎尼酸和绿原酸的含量最高分别达1 530 mg/100 g和700 mg/100g.以60%乙醇为提取剂,在固液比1∶30,50℃超声作用下提取1 h可高效提取有效成分,绿原酸和二咖啡酰奎尼酸的提取率分别达90.3%和91.6%.提取液经大孔树脂CN300用40%～50%和50%～70%的乙醇溶液洗脱,可有效分离提取液中的二咖啡酰奎尼酸与绿原酸,再经乙酸乙酯萃取纯化后可分别得到绿原酸和二咖啡酰奎尼酸产品.

In this work,the natural compounds of the invasive plant Solidago Canadensis are studied,and the contents of dicaffeoylquinic acids and chlorogenic acid are analyzed. The separation and purification process are discussed. The contents of dicaffeoylquinic acids and chlorogenic acid are the highest in flower and leaf of Solidago Canadensis. The contents of dicaffeoylquinic acids and chlorogenic acid reach 1 530 mg/100 g and 700 mg/100 g in the bud of Solidago Canadensis respectively. The extraction rates of chlorogenic acid and dicaffeoylquinic acids respectively reach 90.3%and 91.6% under the optimal extraction conditions：ethanol concentration 60%,extraction temperature 50 ℃,extraction time 1 h,and solid-liquid ratio 1∶30 g/m L. The dicaffeoylquinic acids and chlorogenic acid are effectively extracted by the macroporous resin CN300 with 40%～50% and 50%～70% ethanol solution,and then having been extracted by ethyl acetate,chlorogenic acid and dicaffeoylquinic acids are obtained respectively.