昆虫细胞分类技术的应用与发展

The application and development of insect cell classification techniques

昆虫细胞杆状病毒表达系统作为四大表达系统之一，已经被广泛应用于生物制药领域，作为该表达系统的支撑基础，昆虫细胞开始成为继哺乳动物细胞之后的另一种新型细胞基质，在疫苗及生物技术产品领域得到开发应用。然而，昆虫细胞与哺乳动物细胞在细胞特性等方面存在显著差异，建立适合昆虫细胞特性的质量控制方法已经成为生物制药领域必须要解决的问题。此文仅就昆虫细胞鉴定方法研究进展做一综述，为开发及建立昆虫细胞个性化鉴别方法提供参考。

Baculovirus-insect cell expression system as one of the four major expression systems has been widely used in biopharmaceutical industry. As the basis support of this expression system, insect cells begin to become another new cell matrix after mammalian cells, and are developed and applied in the field of vaccines and biotechnology products. However, there are significant differences between insect cells and mammalian cells in terms of cell characteristics, so establishment of a quality control method suiting characteristics of insect cells has become a problem which must be solved in biopharmaceutics. This article mainly reviews the research progress of insect cell identification methods, and provides reference for the development and establishment of individual identification methods of insect cells.