摘要
A novel sensitive semi-quantitative virus detection technique was developed using the respiratory syncytial virus(RSV) as an example, through dark-field light scattering imaging of the surface state of the virusinvaded host cells. In this method, anti-RSV-antibody modified gold nanoparticles(Au NPs) could bind with the invading virus on the cell membrane of the infected host cells through the specific antibody-antigen binding. Then,the host cells could be imaged by the localized surface plasmon resonance light scattering properties of Au NPs under a dark-field light scattering microscopy, which could be further used to semi-quantify the invading virus.
A novel sensitive semi-quantitative virus detection technique was developed using the respiratory syncytial virus （RSV） as an example, through dark-field light scattering imaging of the surface state of the virus- invaded host cells. In this method, anti-RSV-antibody modified gold nanoparticles （AuNPs） could bind with the invading virus on the cell membrane of the infected host cells through the specific antibody-antigen binding. Then, the host cells could be imaged by the localized surface plasmon resonance light scattering properties of AuNPs under a dark-field light scattering microscopy, which could be further used to semi-quantify the invading virus.
基金
supported by the National Basic Research Program of China(2011CB933600)
Chongqing Fundamental and Advanced Research Project(cstc2013jcyj A50008)
the Fundamental Research Funds for the Central Universities(XDJK2015B029)
