摘要
Worker honeybee pierces animal or human skin with its ultra-sharp stinger and injects venom to defend itself. The insertion behavior is a painless transdermal drug delivery process. In this study, Apis cerana cerana worker honeybee was chosen as the research object. The geometry and structure of the stinger were observed by the Scanning Electron Microscope （SEM）. High-speed video imaging technique was adopted to observe the stinger insertion and pull behavior of honeybee. The skin insertion, pull-out, in-plane buckling and out-of-plane bending forces of honeybee stinger were tested by a self-developed mechanical loading equipment. Results showed that the honeybee stinger pierces directly into skin without frequent vibration. The pull-out force （average 136.04 mN） was two orders of magnitude higher than the penetration force （average 1.34mN）. Compared with the penetration force, the in-plane buckling force （average 6.72 mN） was in the same order of magnitude. The result of out-of-plane bending test showed that the stinger was elastic and it could recover after bending. The excellent geometry and structure of honeybee stinger will provide an inspiration for the further improved design ofmicroneedle-based transdermal drug delivery system.
Worker honeybee pierces animal or human skin with its ultra-sharp stinger and injects venom to defend itself. The insertion behavior is a painless transdermal drug delivery process. In this study, Apis cerana cerana worker honeybee was chosen as the research object. The geometry and structure of the stinger were observed by the Scanning Electron Microscope （SEM）. High-speed video imaging technique was adopted to observe the stinger insertion and pull behavior of honeybee. The skin insertion, pull-out, in-plane buckling and out-of-plane bending forces of honeybee stinger were tested by a self-developed mechanical loading equipment. Results showed that the honeybee stinger pierces directly into skin without frequent vibration. The pull-out force （average 136.04 mN） was two orders of magnitude higher than the penetration force （average 1.34mN）. Compared with the penetration force, the in-plane buckling force （average 6.72 mN） was in the same order of magnitude. The result of out-of-plane bending test showed that the stinger was elastic and it could recover after bending. The excellent geometry and structure of honeybee stinger will provide an inspiration for the further improved design ofmicroneedle-based transdermal drug delivery system.
基金
Acknowledgment This research is financially supported by the National Nature Science Foundation of China （No. 51205423, No. 51205138, and No. 51575543）.
