驱铅治疗后铅中毒患者CD3γ和CD3δ及CD3ε链mRNA水平的变化

Changes of CD3γ,CD3δ and CD3ε chains mRNAs in lead-poisoned patients after chelate treatment

目的了解驱铅治疗后铅中毒患者外周血中T细胞受体信号转导分子CD3γ、CD3δ、CD3ε链表达情况。方法采用实时荧光定量PCR分别检测9例健康人及16例铅中毒患者临床上进行驱铅治疗前后外周血单个核细胞中CD3γ、CD3δ、CD3ε链mRNA水平,以β2微球蛋白(β2M)基因为内参基因,根据相对定量公式2-△Ct×100%计算各基因表达水平。采用Spearman秩相关分析健康人和铅中毒患者驱铅治疗前后CD3γ、CD3δ、CD3ε链mRNA水平相关性。结果铅中毒患者驱铅治疗前后CD3γ链mRNA水平与健康人相比无明显差异;驱铅治疗前后CD3δ链mRNA水平均显著低于健康人,驱铅治疗后CD3δ链mRNA水平较治疗前更低;驱铅治疗前CD3ε链mRNA水平与健康人相比显著升高,驱铅治疗后CD3ε链mRNA水平有所恢复。健康人中CD3γ链与CD3δ链、CD3γ链与CD3ε链、CD3δ链与CD3ε链均无相关性;铅中毒患者驱铅治疗前CD3γ链与CD3δ链,CD3γ链与CD3ε链,CD3δ链与CD3ε链均呈显著正相关,驱铅治疗后仅CD3δ链与CD3ε链呈显著正相关。结论铅中毒患者CD3γ、CD3δ、CD3ε链mRNA水平呈不同趋势的改变,提示铅中毒后对机体免疫系统有一定影响并且在不同基因之间存在差异;驱铅治疗后体内铅负荷降低,CD3δ链表达水平仍然与健康人不同,说明驱铅治疗后机体的免疫功能仍存在异常。

Objective To investigate the expressions of T cell receptor（ TCR） signaling CD3γ,CD3δ and CD3ε chains in T cells from peripheral blood mononuclear cells（ PBMCs） in lead-poisoned workers after chelate treatment. Methods The expression levels of CD3γ,CD3δ and CD3ε mRNAs in PBMCs from 16 lead-poisoned workers before and after chelate treatment and 9 healthy individuals were detected by real-time quantitative PCR. The β2-microglobulin gene（ β2M） was used as an endogenous reference. Relative mRNA levels were calculated by the 2- △Ct× 100%. Spearman correlation analysis was used to estimate the correlations of different gene expression levels between healthy individuals and the patients as well as before and after the treatment. Results The CD3γ mRNA level in the lead-poisoned workers before and after chelate treatment has no significant difference as compared with that of the healthy individuals. The expression levels of CD3δ mRNA in the lead-poisoning workers before and after chelate treatment were remarkably lower than that of the healthy individuals.After chelate treatment,the CD3δ mRNA level in the lead-poisoned workers was lower than that before treatment. The CD3εmRNA level in the lead-poisoned workers before treatment was obviously higher than that of the healthy individuals,while the expression level of CD3ε mRNA decreased after chelate treatment. There were no evident correlations between CD3γ and CD3δ,CD3γ and CD3ε,CD3δ and CD3ε mRNAs,while CD3γ and CD3δ,CD3γ and CD3ε,CD3δ and CD3ε mRNAs had markedly positive correlations in the lead-poisoned workers before chelate treatment,and only CD3 δ and CD3 ε had a significantly positive correlation after the treatment. Conclusion The expression levels of CD3γ,CD3δ and CD3ε mRNAs in the lead-poisoned workers showed varied alterations,which indicated that lead poisoning had an impact on human immunity system and affected different genes in different ways. After chelate treatment,CD3δ mRNA level was still different from that of healthy individuals,which indicated that the function of the immunity system was still abnormal.