IGF-1和MMP-9检测在溃疡性结肠炎的临床应用价值研究

The clinical application value research of IGF-1 and MMP-9 in ulcerative colitis

[目的]检测溃疡性结肠炎（UC）患者胰岛素样生长因子-1（IGF-1）和基质金属蛋白酶-9（MMP-9）表达变化,并探讨其相关的临床意义。[方法]收集2013年2月-2015年8月我院消化内科诊治的溃疡性结肠炎患者54例,另取同期我院健康体检者40例作为正常对照。ELISA法检测IGF-1和MMP-9表达。[结果]对照组IGF-1表达为（113.64±32.52）ng/ml,显著高于UC组的（68.57±21.38）ng/ml（P〈0.05）。对照组MMP-9表达为（16.15±3.23）ng/ml,显著低于UC组的（24.36±5.74）ng/ml（P〈0.05）。缓解期患者IGF-1表达为（90.52±28.18）ng/ml,显著高于活动期患者的（45.32±17.52）ng/ml（P〈0.05）。缓解期患者MMP-9表达为（18.09±3.07）ng/ml,显著低于活动期患者的（33.42±4.72）ng/ml（P〈0.05）。内镜活动性分级Ⅰ、Ⅱ、Ⅲ级溃疡性结肠炎患者之间IGF-1和MMP-9表达存在显著性差异（P〈0.05）。其中IGF-1表达Ⅰ级患者高于Ⅱ级患者（P〈0.05）,Ⅱ级患者又高于Ⅲ级患者（P〈0.05）;其中MMP-9表达Ⅰ级患者低于Ⅱ级患者（P〈0.05）,Ⅱ级患者又低于Ⅲ级患者（P〈0.05）。[结论]IGF-1和MMP-9异常表达可能在溃疡性结肠炎发病机制中发挥着重要作用,检测二者有可能作为评价溃疡性结肠炎患者病情活动性及严重程度的指标。

[Objective]To detect the expression changes of IGF-1and MMP-9in ulcerative colitis（UC）,and to explore the relative clinical application value.[Methods]54 UC patients who were treated in our hospital and 40 healthy person were enrolled in this study from February,2013 to August,2015.The expression of IGF-1and MMP-9 was detected by ELISA analysis.[Results]The expression of IGF-1 was113.64 ±32.52ng/ml in control group,which was significantly higher than that of 68.57 ±21.38ng/ml in UC group（P〈0.05）.The expression of MMP-9was 16.15±3.23ng/ml in control group,which was significantly lower than that of 24.36±5.74ng/ml in UC group（P〈0.05）.The expression of IGF-1was90.52±28.18ng/ml in remission stage of the UC patients,which was significantly higher than that of45.32±17.52ng/ml in active stage（P〈0.05）.The expression of MMP-9was 18.09±3.07ng/ml in remission stage,which was significantly lower than that of 33.42±4.72ng/ml in active stage（P〈0.05）.There was significant differences of IGF-1and MMP-9expression among endoscopic activity gradeⅠ,ⅡandⅢ patients（P〈0.05）.The expression level of IGF-1was higher in gradeⅠthan that in gradeⅡ（P〈0.05）,and in gradeⅡ,the level was higher than that in gradeⅢ（P〈0.05）.The expression of MMP-9was lower in gradeⅠthan that in gradeⅡ（P〈0.05）,and in gradeⅡ,the level was lower than that in gradeⅢ（P〈0.05）.[Conclusion]The abnormal expression of IGF-1and MMP-9 may play an important role in the pathogenesis of UC,and which may be used as an indicator to evaluate the activity and severity of UC.