香蕉枯萎病菌假定分泌蛋白SP10的功能分析

Functional Analysis of the Putative Secretory Protein SP10 in the Fungal Pathogen Fusarium oxysporum f. sp.cubense Causing Fusarium wilt.

为了研究尖孢镰刀菌古巴专化型假定分泌蛋白基因SP10的在致病过程的作用,构建SP10基因敲除菌株,分析SP10敲除菌株的表型特征。结果表明:SP10基因敲除菌株在菌丝生长、产孢和菌落形态等方面无明显变化,但SP10基因敲除菌株对巴西蕉的致病性减弱;在接种30 d后,采用共聚焦显微镜显微观察香蕉根系,结果显示,SP10基因敲除菌株可在根系维管束中定殖。上述结果表明,假定的分泌蛋白SP10参与调控尖孢镰刀菌对香蕉的致病性,但不参与该病原菌生长和发育的调控。此结果为进一步研究尖孢镰刀菌分泌蛋白的功能奠定了基础。

To investigate the functions of the putative secretory protein SP10 in the banana fungal pathogen Fusarium oxysporum f. sp. cubense（Foc） during infection of banana, the ΔSP10 deletion strains were constructed.The ΔSP10 deletion strains had no alterations in terms of growth and development, but the pathogenicity against the banana（Musa spp. cv. Brazil） was reduced. Microscopic observation of banana roots using the confocal laser scanning microscope 30 d post inoculation revealed that the ΔSP10 deletion strain could colonize the vascular vessels of banana roots. These results provide a basis for further study of the functions of secretory proteins in F.oxysporum.