外源ABA对金线兰抗氧化酶活性及其同工酶的影响

Effect of Exogenous ABA on Activities and Isozymes of Antioxidant Enzymes in Anoectochius roxburghii(Wall.)Lindl.

以金线兰组培苗为试材,采用提取粗酶液的方法,研究外源脱落酸(ABA)对金线兰超氧化物歧化酶(SOD)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)、过氧化氢酶(CAT)4种抗氧化酶活性及其同工酶的影响。结果表明:高浓度ABA的长时间处理对提高SOD和POD活性较为显著;低浓度ABA处理下APX活性变化幅度较大,其中高浓度ABA处理24h时APX活达到最大值;不同浓度ABA处理下CAT活性均呈"M"型的变化趋势。同工酶电泳分析发现,不同浓度的ABA处理,在一定时间范围内均可诱导产生新的POD和APX酶带,而不诱导新的SOD和CAT酶带的表达。

With the tissue cultured seedling of Anoectochilus roxburghii（Wall.）Lindl.as test material,using crude extract method,the effect of exogenous ABA on activities of antioxidant enzymes（SOD,POD,APX,and CAT）and isozymes was studied.The results showed that long time high concentration of ABA treatment on the activity of SOD and POD was significant;APX activity under low concentration of ABA treatment change was bigger,and APX enzyme activity reached the maximum under high concentration of ABA treatment for 24hours;and CAT showed ‘M＇shifting trend under different concentration of ABA.By the analysis of isozymes,different concentration of ABA could induce new bands of POD and APX in specific time,but no new expression of SOD and CAT.