广西野生灵芝菌株的遗传多样性分析

Genetic Diversity Analysis of Wild Ganoderma Strains Collected From Guangxi

以10个广西野生灵芝菌株为试材,采用酯酶同工酶分析和拮抗试验的方法,研究供试菌株的遗传多样性。结果表明：广西野生灵芝具有丰富的遗传多样性。试验共检测到22条谱带,10个菌株间的谱带均有差异。各菌株间的遗传相似系数在0.36～0.95;在相似系数为0.55时,10个菌株可以分为3类,即菌株C3（红芝）为一类,菌株C9（白芝）为一类,C1、C2、C4、C5、C6、C7、C8、C10（黑芝）为一类;在相似系数为0.64时,黑芝类又可分为2类,即C1、C2、C4、C8、C10为一类,C5、C6、C7为一类。菌株C4与C10、C5与C7间的遗传相似系数大于0.90,亲缘关系很近,且这2对菌株间无拮抗,说明菌株C4与C10为同一菌株,C5与C7也为同一菌株。

Genetic diversity of ten wild Ganodermastrains collected from Guangxi was analyzed by using the technique of esterase isozyme analysis and antagonistic experiment.The results showed that there were rich genetic polymorphisms within the wild Ganodermain Guangxi.22 bands were determined in the trail,and the bands among the strains were different.Cluster analysis demonstrated that the similarity coefficients ranged from 0.36 to 0.95 among the ten strains and were divided into 3groups at the similarity coefficient of 0.55,which was Ganoderma lingzhi（strain C3）,Ganoderma lucidum（strain C9）and Ganoderma atrum（strains C1,C2,C4,C5,C6,C7,C8,C10）.The group of G.atrum could be divided into 2subgroups（subgroup A included strains C1,C2,C4,C8 and C10,subgroup B included strains C5,C6 and C7）at the similarity coefficient of 0.64.Strains C4 and C10,as well as strains C5 and C7could be considered as different isolates in the same strain,as they had high similarity coefficients above of 0.90 and without antagonistic activity between both strains.