银花平感颗粒抗菌作用的实验研究

An experimental study on antibacterial effects of Yinhua Pinggan granule

目的观察中药银花平感颗粒对金黄色葡萄球菌（金葡菌）的抗菌作用。方法采用小鼠腹腔注射金葡菌液致小鼠感染模型，连续给予银花平感颗粒2．0、1．0、0．5mg／kg组中药银花平感颗粒5000．0、2500．0、1250．0mg·kg^-1·d^-1共7d，以双黄连口服液和清热解毒口服液作为阳性对照药物；记录各组小鼠的死亡率；同时采用体外抗菌活力测定实验，观察银花平感颗粒的体外抗菌活力变化。结果银花平感颗粒对金葡菌感染大鼠具有明显的保护作用，且呈量效正相关关系[模型组大鼠死亡率为88．9％（16／18），双黄连口服液组为81．2％（13／16），清热解毒121服液组为43．8％（7／16），银花平感颗粒0．5、1．0、2．0mg／kg组死亡率为75．O％（12／16）、50．0％（8／16）、31．2％（5／16）]。体外抗菌活力测定表明，银花平感颗粒0．117mg／L即对大肠杆菌、肺炎球菌、肺炎克雷伯菌、乙型链球菌、痢疾杆菌、流感杆菌、金葡菌都具有显著的抗菌作用，其抗菌药物浓度明显低于其他中药和青霉素，抗菌谱也宽。结论银花平感颗粒对金葡菌感染小鼠具有明显体内保护作用；且银仡平感颗粒抗菌谱广，对临床上常见致病菌均有抑制作用。

Objective To observe the antimierobial effect of Yinhua Pinggan granule （YHPG） on staphylococcus aureus. Methods Infection models of mice were established by intraperitoneal injection of staphylococcus aureus liquid, and the mice in the YHPG 2.0, 1.0 and 0.5 mg/kg group were sequentially administrated YHPG orally at doses of 5 000.0, 2 500.0, 1 250.0 mg·kg^-1·d^-1 for 7 days respectively. The Shuanghuanglian （SHL） and Qingrejiedu （QRJD） oral liquids were used as the positive control drugs. The mortality was recorded in each group. Meanwhile, the changes in the antibacterial activity of YHPG were observed by the antibacterial activity assay experiment in vitro. Results The protective eft^ct of the YHPG on mice infection of staphylococcus aureus was remarkable, representing a dose dependent manner. The mortality in the model group, SHL, QRJD groups and 3 YHPG （0.5, 1.0 and 2 mg/kg） subgroups were 88.9% （16/18）, 81.2% （13/16）, 43.8% （7/16）, 75.0% （12/16）, 50.0% （8/16）, 31.2% （5/16）, respectively. The antibacterial activity assay in vitro showed that YHPG 0.117 mg/L possessed significant activity on escherichia coli, streptococcus pneumoniae, pneumonia grams klebsiella, beta streptococcus, bacillus dysenteriae, influenza bacillus, staphylococcus aureus bacteria, YHPG antibacterial drug concentration was significantly lower than the concentrations of other traditional Chinese medicines （TCM） and penicillin, and its antibacterial spectrum was broad. Conclusion YHPG has significant protective effects on mice infected with staphylococcus aureus, indicating that YHPG with antibacterial broad-spectrum has inhibitory effects on common clinical pathogenic bacteria.