温度对生物膜—活性污泥复合工艺硝化特性及硝化菌种群的影响

Influence of Temperature on Nitrification Characteristics and Nitrifying Bacteria Community in Integrated Fixed Film— Activated Sludge Process

通过模拟污水厂水温运行小试反应器，利用高通量测序技术结合水质指标对生物膜活性污泥复合工艺的硝化特性和硝化种群结构进行研究。结果表明，泥膜两相优势AOB及NOB均分别为亚硝化单胞菌属和硝化螺旋菌属；在高温段（20-30 ℃），泥相内硝化细菌平均数量高于膜相，泥相对系统硝化贡献率平均达85%，硝化作用主要发生在泥相；在低温段（10-15 ℃），膜相内硝化细菌平均数量高于泥相，泥相氨氧化速率降低约60%，而膜相对系统的硝化贡献率从11%增加至38%，填料的投加确保了工艺在低温时稳定的硝化效果；运行实际工艺时可在水温低于15 ℃时投加填料增强硝化，水温高于20 ℃时移除填料节约运行成本。

An Integrated Fixed Film Activated Sludge process reactor was operated at different temperatures and Illumina MiSeq sequencing and batch test were used to reveal the nitrification characteristic of the process. The results show the most predominant AOB and NOB in IFAS system are Nitrosomonas and Nitrospira. In the high temperature period （ 20-30℃ ） , nitrifying bacteria are more abundant in sludge than biofilm, nitrification is mainly happened in sludge with a average 85% nitrification contribution rate. While the AOR of sludge decreased 60% and nitrifying bacteria are more abundant in biofilm than sludge in low temperature period （ 10-15℃）. And average nitrification contribution rate of biofilm increase from 11% to 38%. Considering the enhanced nitrification or operating cost, the WWTP can make a decision by operation temperature selectively.