四环素耐药基因在养猪场猪粪和土壤中的分布研究

The Distribution of Tetracycline Resistance Gene in Swine Manure and Soil of Hoggery

该文采用PCR方法研究了养猪场中猪粪和土壤中耐四环素乳糖发酵型肠杆菌（TR-LFE）中的tet-R分布和多样性。结果表明,在分离的258株耐四环素乳糖发酵型肠杆菌中,共检测到191株含有tet-R基因,占74.03%。各样品的耐四环素乳糖发酵型肠杆菌中tet-R基因的检出率范围为3.12%~96.87%。根据检出频率的大小,各类tet-R基因的排列顺序为：tet（A）,tet（B）,tet（M）,tet（G）,tet（C）,tet（D）,tet（S）,所有样品都没有检测到tet（O）和tet（X）。

In this paper,PCR was applied to analyze the distribution and diversity of tetracycline resistant genes（tet-R gene）in tetracycline resistant Lactose-fermenting Enterobacteriaceae（TR-LFE）separated from swine manureand soil in hoggery.The research results showed that 191 of the 258 strains of TR-LFEs were detected to be tet-Rgene positive,accounting for 74.03%.The rates of tet-R gene positive TR-LFE were in the range of 3.12%~96.87%.Based on the detection frequency of tet-R genes,the general rank of tet-R genes was set out as follows：tet（A）,tet（B）,tet（M）and tet（G）and tet（C）and tet（D）and tet（S）.All the samples were not detected to be tet（O）andtet（X）.