静息细胞转化北沙参中总香豆素的研究

Study on total coumarins of Glehnia littoralis by resting cells transformation

目的优化极地真菌S-3-66-E1转化北沙参中总香豆素的条件。方法采用静息细胞转化法,考察了生物转化条件(菌体密度、底物浓度、温度、pH、摇床转速、金属离子)对转化体系的影响,并通过正交实验优化工艺参数,利用HPLC检测总香豆素的转化情况。结果确定摇瓶转化的最优条件:培养温度15℃,摇床转速240r/min,菌体密度60g·L^(-1),底物浓度0.1g·L^(-1),pH值5.9,转化时间24h,在此条件下总香豆素的转化量趋近完全。结论采用静息细胞法转化北沙参中总香豆素的工艺稳定可行。

Objective To optimize the transformation conditions of total coumarins fromGlehnia littoralis by polar fungus S-3-66-E1.Methods The effects of biological transformation conditions（cell concentration,substrate concentration,temperature,pH,rotation speed,metal ion）were optimized by orthogonal design,and detected by high performance liquid chromatography（HPLC）.Results The optimal transformation conditions were determined as follows：culture temperature 15℃;rotation speed 240r/min;cell concentration 60g·L-1;substrate concentration 0.1g·L-1;pH 5.9;conversion time 24 h,which the amount of total coumarins transformed completely.Conclusion The transformation condition of the resting cell of total coumarins fromGlehnia littoralis by polar fungus S-3-66-E1 was stable and feasible.