附子新型炮制品对阳虚小鼠耳廓微循环的影响

Effects of Modern Processed Products of Aconiti Lateralis Radix Praeparata on Auricular Microcirculation in Mice with Yang Deficiency

目的探索附子新型炮制品对阳虚小鼠耳廓微循环的影响。方法实验设空白组、模型组、4个炮制品给药组[黑顺片组、高温炮制品组、高压炮制品组、微波炮制品组,每组设高、中、低3个剂量(0.75,3.00,5.63 g·kg?1)],连续灌胃给药9 d,于末次给药24 h后,用显微测微尺测量耳廓静脉与动脉血管管径及毛细血管网交叉点数等,并根据炮制品生物碱含量分析其量效关系。结果与模型组比较,除微波炮制品0.75 g·kg?1组动脉管径外,各给药组小鼠耳廓动脉与静脉管径均有不同程度增加,毛细血管网交叉点数逐渐变大(P<0.05),并与剂量大小呈正相关;与黑顺片组比较,高压炮制品5.63 g·kg?1剂量组静脉管径显著增加(P<0.05);4个炮制品给药组相同剂量比较,高压炮制品组动脉管径、静脉管径和毛细血管网交叉点数值均最高。结论附子高压炮制品能显著增强阳虚小鼠耳廓微循环,其作用优于黑顺片、高温炮制品和微波炮制品。

OBJECTIVE To explore the effects of processed products of Aconiti Lateralis Radix Praeparata on auricular microcirculation in mice with Yang deficiency. METHODS The mice were randomly divided into 14 groups： the control group, the model group, experimental groups（Heishunpian groups, high temperature groups, high pressure groups, microwave treatment group）, each set were divided into high, medium and low drug dosage（0.75, 3.00, 5.63 g·kg^-1）, all the mice were given intragastric administration for 9 d. Twenty-four hours after the last administration, with the vascular diameter of vein and artery and capillary network cross points were measured by microscopic micrometer. The dose-effect relationship was analyzed according to the content of alkaloids of processed products. RESULTS Compared with the model group, the vascular diameter of vein and artery were increased of the processed products groups except 0.75 g·kg^-1 microwave treatment group, and the capillary network crossover points of the processed products groups were also increased（P〈0.05）. The effect was positively correlated with the dose. Compared with Heishunpian group, the vascular diameter of artery was significantly increased of high-pressure processed products with the dose of 5.63 g·kg^-1（P〈0.05）. Comparing 4 processed products administered group with the same dose, the vascular diameter of vein, artery and capillary network cross points of high pressure processed products were the highest. CONCLUSION The high pressure processed products could significantly enhance the microcirculation of mice with Yang deficiency, its effect is better than Heishunpian, high temperature processed products, or microwave processed products.