双肾上腺皮质激素样激酶-1与细胞周期蛋白D1在结直肠癌组织中的表达及临床意义

Expression and clinical significance of doublecortin and CaM kinase-like-1 and cyclin D1 in colorectal carcinomas

目的：探讨双肾上腺皮质激素样激酶-1（EgJLKl）与细胞周期蛋白D1（cyclin D1）在结直肠癌组织中的表达及其临床意义。方法：采用免疫组织化学S-ABC法检测59例结直肠癌组织和28例结直肠良性病变组织中DCI。K1及cyclinD1的表达及分布。结果：DCLK1及cyclin D1在结直肠癌组织中的表达明显高于结直肠良性病变组织，平均光密度值（AOD）为0．423±0．021及0．386±0．042；而在结直肠良性病变组织中其阳性反应的平均光密度值为0．267±0．053及0．21l±0．026，差异具有统计学意义。DCLK1及cyclinD1的阳性表达与患者的年龄、性别、肿瘤分化程度和DUCKs分期无关，而与组织良性或恶性、肿瘤的大小及淋巴结转移相关。结论：结直肠癌组织中DCLKl及cyclinD1的高表达可能与结直肠癌的发生、发展、转移相关，可作为结直肠癌转移及预后监测的一项重要指标。

Objective： To explore the expression and clinical significance of doublecortin and CaM kinasedike-1 （DCLK1） and cyclin D1 in coloreetal carcinomas. Methods： Expression and distribution of DCLK1 and cyclin D1 were detected by S-ABC immunohistochemistry in 59 colorectal carcinomas and 28 benign lesions on colorectus. Results： in the colorectal carcinomas, DCLK1 and cyclin D1 expression was up-regulated, AOD were 0. 423 ± 0. 021 and 0. 386±0. 042; in benign lesions of colorectus, AOD were 0. 267±0. 053 and 0. 211±0. 026, and the difference was statistically significant. There was no relation among positive-reaction of DCLK1, cyclin D1 and the patient＇s age, sexuality, tumor differentiation and DUCKs stage. However, they were correlated with histologic type, tumor diameter and lymph node metastasis. Conclusion： I）CLK1 and cyclin D1 over-expression may play a role in the carcinogenesis, development metastasis and relapse of colorectal cancer. They could be used as a significant monitoring marker of metastasis and prognostic supervision of colorectal cancer in clinical practice.