冬虫夏草菌丝体多糖对免疫性肝损伤小鼠的保护作用

Protective effect of mycelial polysaccharides from Cordyceps sinensis on immunological liver injury in mice

目的探讨冬虫夏草菌丝体多糖对小鼠肝损伤模型的保护作用。方法采用卡介苗联合脂多糖(BCG+LPS)建立小鼠免疫性肝损伤模型。60只NIH小鼠随机均分为正常对照组,模型组,菌丝体多糖高、中、低剂量(100、50、25mg/kg)组和联苯双酯(150mg/kg)组,每组10只。检测小鼠血清ALT、AST及NO水平;制备肝组织匀浆,检测IL-1β、TNF-α含量;Real-time PCR检测小鼠肝组织IL-6、i NOS基因m RNA表达水平。结果在小鼠免疫性肝损伤模型中,高、中剂量冬虫夏草菌丝体多糖可明显降低血清ALT、AST水平(P<0.01),高剂量冬虫夏草菌丝体多糖可明显降低血清NO和肝组织IL-1β、TNF-α含量(P<0.01)。与模型组相比,高、中剂量冬虫夏草菌丝体多糖可明显下调IL-6、i NOS m RNA的表达(P<0.01)。结论冬虫夏草菌丝体多糖对免疫性肝损伤具有一定的保护作用。

Objective To explore the protective effects of mycelial polysaccharides from Cordyceps sinensis（MPCS） on BCG＋LPS-induced liver injury in mice. Methods The immunological liver injury mice model was reproduced by giving bacillus Calmette-Guerin（BCG） and lipopolysaccharide（LPS）. Sixty NIH mice were randomly assigned into 6 groups（10 each）： normal control group, model group, mycelium polysaccharide in high（100mg/kg）, medium（50mg/kg） and low（25mg/kg） dose group, and bifendate（150mg/kg） treatment group. The serum transaminase levels of alanine ALT and AST were assayed with ELISA, nitric oxide（NO） in serum was measured by nitrate reductase method, and the liver homogenate was prepared for the determination of the contents of interleukin-1β（IL-1β） and tumor necrosis factor-α（TNF-α）. The m RNA expression levels of IL-6 and i NOS in hepatic tissue were assessed using RT-PCR. Results In the mice of immunological liver injury, mycelial polysaccharides from Cordyceps sinensis obviously lowered the serum ALT and AST levels（P〈0.01）, high dose MPCS significantly reduced the serum NO and liver tissue IL-1β and TNF-α levels（P〈0.01）. Compared with the model group, high and medium dose MPCS significantly reduced the expression levels of IL-6 and i NOS m RNA in hepatic tissues（P〈0.01）. Conclusion MPCS shows a certain protective effect on immunological liver injury induced by BCG plus LPS in mice.