基于关联规则挖掘治疗多发性硬化所用中药对患者T细胞亚群的影响

Effect of Chinese Herbs Used in Treating Multiple Sclerosis on T Subsets Using Association Rules

目的应用关联规则和统计学方法分析李涛教授治疗多发性硬化(MS)所用中药对MS患者外周血T细胞亚群的影响,为优化处方提供依据。方法整理中国中医科学院西苑医院病例采集系统所录入的门诊及病房MS患者的资料。采用SPSS 17.0和SPSS Clementine 12.0软件Aprior模块分析中药与T细胞亚群变化的关系。结果柴胡、白芍、枳壳、白术、甘草为最常用药物组合,常加附子和土茯苓。附子关联于Th1细胞下降(置信度83.78%,支持度36.26%),Th1细胞下降关联于附子(置信度71.26%,支持度36.26%),附子与Th1细胞下降存在明显关联关系。柴胡、白芍、枳壳、白术、甘草、附子组方可降低MS患者Th1细胞,升高Th2细胞,差异均有统计学意义(P<0.01)。结论柴胡、白芍、枳壳、白术、甘草、附子和土茯苓组合能降低MS患者外周血中Th1细胞,升高Th2细胞,其中附子对Th1、Th2的上述改变起重要作用。

Objective To analyze the effect of Chinese herbs used by Prof.LI Tao on peripheral blood T subsets in treating multiple sclerosis（MS）by using association rules and statistical methods,thereby providing evidence for optimizing prescriptions.Methods Data of MS inpatients and outpatients recorded by data collecting system,Xiyuan Hospital,China Academy of Chinese Medical Sciences were resorted.The relationship between Chinese herbs and T cell subsets were analyzed using SPSS17.0 and Aprior module in SPSS Clementine 12.0.Results Radix bupleuri,Radix Paeoniae alba,Fructus Aurantii,Atractylodes,and Radix Glycyrrhizae were most commonly used herbal combinations.Radix Aconiti lateralis preparata and Rhizoma Smilacis glabrae were often added.Radix Aconiti lateralis preparata was associated with decreased Th1 cells（confidence level 83.78%,supportive level 36.26%）.Decreased Th1 cell was associated with Radix Aconiti lateralis preparata（confidence level 71.26%,supportive level 36.26%）.Radix Aconiti lateralis preparata was obviously associated with decreased Th1 cells.Radix Bupleuri,Radix Paeoniae alba,bitter orange,Atractylodes,Radix glycyrrhizae,and Radix Aconiti lateralis preparata could reduce peripheral blood Th1 subsets of MS patients and elevate Th2 subsets（all P〈0.01）.Conclusions The herbal combination of Radix Bupleuri,Radix Paeoniae alba,Fructus Aurantii,Atractylodes,Radix Glycyrrhizae,Rhizoma Smilacis glabrae,and Radix Aconiti lateralis preparata could lower peripheral blood Th1 cells and elevate Th2 cells,and prevent the relapse of MS possibly by reducing Th1 cells and elevating Th2 cells.Especially Radix Aconiti lateralis preparata played important roles in aforesaid changes of Th1 and Th2.