舒胃汤对FD大鼠小肠深部肌间神经丛胆碱能神经-Cajal间质细胞-平滑肌网络结构损伤的影响

Effect of Shuwei Decoction on Enteric Nervous System-Interstitial Cells of Cajal-Smooth Muscle Network Structure Injury in Deep Muscle Nerve Plexus of Functional Dyspepsia Rats

目的观察功能性消化不良(FD)大鼠小肠深部肌间神经丛胆碱能神经-Cajal间质细胞(ICC)-平滑肌细胞(SMC)结构的形态学改变和舒胃汤对其的修复作用,探讨舒胃汤治疗FD的机制。方法将72只大鼠随机分为对照组、模型组、舒胃汤低剂量组(简称舒低组)、舒胃汤中剂量组(简称舒中组)和舒胃汤高剂量组(简称舒高组),莫沙比利组(简称西药组),每组12只。舒低组、舒中组、舒高组分别给予舒胃汤0.767、1.534、3.068g/mL,西药组予莫沙必利1.37mg/kg。采用复合病因造模法制造FD肝郁脾虚证大鼠模型,造模后第3日各组给予相应药液,对照组和模型组予以蒸馏水(10 mL/kg),均1日1次,持续14日。第15日处死取小肠组织做免疫荧光双标记、激光扫描共聚焦显微镜和透射电镜观察大鼠小肠深部肌间神经丛胆碱能神经-ICC-SMC结构损伤和舒胃汤对其的修复作用。结果模型组小肠深部肌间神经丛胆碱能神经-ICC-SMC连接结构不完整,ICC相互之间及ICC与SMC之间的联系结构明显损伤。舒高、中、低剂量组胆碱能神经-ICC-SMC网络结构比较完整,ICC相互之间及ICC与SMC之间联系紧密。结论舒胃汤能促进ICC再生和形态的恢复,从而保持胆碱能神经-ICC-SMC结构完整性,改善胃肠运动障碍,对FD有治疗作用。

Objective To observe morphological changes of enteric nervous system（ENS）-interstitial cells of Cajal（ICC）-smooth muscle cell（SMC）structure injury in deep muscle nerve plexus of functional dyspepsia（FD）rats,and the repair of Shuwei Decoction（SD）on it,and to explore its effect on FD.Methods Totally 72 rats were randomly divided into the control group,the model group,the low dose SD group,the medium dose SD group,and the high dose SD group,the Mosapride group,12 in each group.Rats in the low dose SD group,the medium dose SD group,and the high dose SD group were intragastrically fed with SD at 0.767,1.534,3.068 g/mL,respectively.Rats in the Mosapride group were intragastrically fed with Mosapride（1.37 mg/kg）.FD rat model with Gan depression Pi deficiency syndrome（GDPDS）was established using complex pathogenic factors.Corresponding liquors were respectively administered to rats in corresponding groups from the 3rd day after modeling.Distilled water（10 mL/kg）was administered to rats in the control group and the model group,once per day for 14 successive days.Rats were sacrificed and small intestine tissues collected for observing ENS-ICC-SMC structure injury using immunofluorescencedouble labeling,laser scanning confocal microscope,and transmission electron microscope at day 15.Repair of SD on it was also observed.Results ENS-ICC-SMC structure was incomplete,with obvious injury in mutual link of ICC,ICC,SMC,and connecting structure.ENS-ICC-SMC structure was more complete in high,medium,and low dose SD groups,with close link of ICC and SMC.Their connecting structures were in good conditions.Conclusion SD could keep the integrity of ENS-ICC-SMC structure by promoting regeneration and morphology of ICC,thereby,improving gastrointestinal movement disorder and showing therapeutic effect on FD.