针刀对第三腰椎横突综合征兔β内啡肽及脑啡肽含量的影响

Effect of Small Knife Needle onβ-endorphin and Enkephalin Contents of Transverse Process Syndrome of the Third Vertebra

目的通过观察第三腰椎横突综合征兔外周和中枢β内啡肽(β-endorphin,β-EP)和脑啡肽(enkephalin,ENK)含量的变化,探讨针刀治疗第三腰椎横突综合征的镇痛机制。方法选用清洁级日本大耳白兔30只,采用随机数字表法分为正常组、模型组、针刀组、电针组和针刀加电针组5组,每组6只。后4组采用在左侧第三腰椎横突部埋置明胶海绵的方法制作第三腰椎横突综合征模型,针刀组给予针刀干预,电针组给予电针双侧"委中"干预,针刀加电针组给予针刀和电针干预。造模后第28天进行取材,分别检测血浆、肌肉、脊髓和下丘脑中β-EP和ENK含量。结果造模后,模型兔血浆和肌肉中β-EP和ENK含量较正常组升高,脊髓和下丘脑中β-EP和ENK含量较正常组降低,差异均有统计学意义(P<0.05,P<0.01)。各治疗组(针刀组、电针组、针刀加电针组)在治疗后,β-EP和ENK的含量均趋于正常,与模型组比较,肌肉中β-EP含量降低,下丘脑中β-EP及ENK含量升高,差异有统计学意义(P<0.05)。各治疗组间比较,差异无统计学意义(P>0.05)。结论针刀和电针对外周和中枢的β-EP和ENK均有良性的调节作用,针刀较电针更具优势。

Objective To explore the analgesic mechanism of small knife needle for treating transverse process syndrome of the third vertebra（TPSTV）by observing peripheral and central changes ofβ-endorphin（β-EP）and enkephalin（ENK）contents.Methods Totally 30 Japanese white big-ear rabbits of clean grade were divided into5 groups according to random digit table,i.e.,the normal control group,the model group,the small knife needle group,the electroacupunture（EA）group,and the small knife needle plus EA group,6in each group.The TPSTV model was established by inserting apiece of gelatin sponge into the left transverse process of 3rd lumbar vertebrae.Rabbits in the small knife needle group were intervened by small knife needle.Those in the EA group were intervened by EA at bilateral Weizhong（BL40）.Those in the small knife needle plus EA group were intervened by small knife needle and EA at bilateral Weizhong（BL40）.Contents ofβ-EP and ENK in plasma,muscle,spinal cord,and hypothalamus were determined after sample collection at day 28 after modeling.Results Compared with the normal control group,contents ofβ-EP and ENK in plasma and muscle increased significantly,and contents ofβ-EP and ENK inspinal cord and hypothalamus decreased significantly in the model group（P〈0.05,P〈0.01）.Contents ofβ-EP and ENK approximated normal levels in the three treatment groups after respective treatment.Compared with the model group,the content ofβ-EP in muscle decreased,and contents ofβ-EP and ENK in hypothalamus increased in the three treatment groups after respective treatment（P〈0.05）.There were no significant difference among the three treatment groups（P〉0.05）.Conclusions Small knife needle treatment and EA had benign regulation on peripheral and centralβ-EP and ENK in TPSTV rabbits.Small knife needle treatment showed better effect than that of EA.