灵芝发酵液的成分检测及美白与抗衰老功效评价

Analysis of fermentation liquor of Ganoderma lucidum and evaluation of its whitening and anti- senility efficacy

为开发灵芝发酵液在化妆品领域的应用,使用葡萄酒酵母发酵灵芝并测定发酵液中所含有的多糖、多酚、黄酮和多肽含量。通过DPPH自由基清除实验和羟自由基清除实验对灵芝发酵液的抗氧化能力进行检测,并测定其对成纤维细胞存活率的影响以评估其可能在抗衰老方面发挥的作用。最后通过体外酪氨酸酶实验和小鼠黑色素瘤细胞内的酪氨酸酶活性及黑色素合成抑制实验对灵芝发酵液的美白功效进行评价。结果表明,灵芝发酵液富含多糖,质量浓度为9.910 g/L,多酚、黄酮和多肽的质量浓度分别为0.054,0.045和2.931 g/L。体积分数分别为25.89%和42.18%的灵芝发酵液可清除50%的DPPH自由基和羟自由基;体积分数为0.05%-0.2%范围内的灵芝发酵液可有效促进成纤维细胞增殖;灵芝发酵液对酪氨酸酶活性抑制率高达73.22%,其通过抑制酪氨酸酶活性起到抑制黑色素合成的作用。因此灵芝发酵液具有一定的抗衰老和美白功效。

To develop the application of fermentation liquor of Ganoderma lucidum in the field of cosmetics,Saccharomyces ellipsoideus were used to acquire fermentation liquor Ganoderma lucidum,and the content of total polysaccharides,total polyphenols,total flavones and total polypeptides in the fermentation liquor were determined. The scavenging activity of OH·free radicals and DPPH free radicals as well as the influence on fibroblasts viability were measured to evaluate the possible anti- senility effect of the fermentation liquor.Finally,via in vitro tyrosinase activity test and intracellular tyrosinase activity test on melanin synthesis of mouses as well as melanin inhibition test,the evaluation for whitening effect of the fermentation liquor was conducted. Results showed that the fermentation liquor of Ganoderma lucidum contains 9. 910 g / L of total polysaccharides,0. 054 g / L of total polyphenols,0. 045 g / L of total flavones,and 2. 931 g / L of total polypeptides. The fermentation liquor with 25. 89% and 42. 18% volume fraction of fermentation liquor of Ganoderma lucidum could eliminate 50% OH · free radical and DPPH free radical. The fermentation liquor with 0. 05%- 0. 2% volume fraction can promote fibroblasts proliferation effectively. The activity of tyrosinase is inhibited significantly by the fermentation liquor of Ganoderma lucidum and the inhibition rate reaches73. 22%. The fermentation liquor of Ganoderma lucidum could inhibit melanin synthesis by decreasing activity of tyrosinase. The fermentation liquor of Ganoderma lucidum exhibits efficacy for anti- senility and whitening.