乳糖饮水对Sprague-Dawley大鼠糖、脂、尿酸代谢的影响

Influence of Lactose-Sweetened Beverage on Serum Glucose, Lipid and Uric Acid Levels in Sprague-Dawley Rats

目的：探讨不同质量浓度乳糖饮水对Sprague-Dawley（SD）大鼠糖、脂、尿酸代谢的影响。方法：分别以清水,50、100、150 g/L乳糖水饲喂大鼠8周,观察不同时间点大鼠的能量摄入及体质量等一般状态指标。取大鼠血清检测血糖（glucose,GLU）、尿酸（uric acid,UA）、甘油三酯（triglyceride,TG）、总胆固醇（total cholesterol,TC）、高密度脂蛋白胆固醇（high density lipoprotein cholesterol,HDL-C）、低密度脂蛋白胆固醇（low density lipoprotein cholesterol,LDL-C）水平。结果：与正常组相比,150 g/L乳糖组大鼠的能量摄入显著降低,且于实验第1、2周体质量显著降低（P〈0.05）。各剂量乳糖组大鼠的GLU水平均高于正常组（P〈0.05或P〈0.01）。50、100 g/L乳糖组大鼠的UA水平低于正常组（P〈0.05或P〈0.01）,150 g/L乳糖组大鼠的UA水平于第1周一次性升高（P〈0.05）。100 g/L乳糖组大鼠的TG水平于第6周显著升高（P〈0.05）,150 g/L乳糖组大鼠的TG水平于第1~3周显著升高且TC水平于第2、3、7周显著升高（P〈0.05或P〈0.01）。乳糖组大鼠的LDL-C水平均显著或极显著升高（P〈0.05或P〈0.01）。结论：不同质量浓度乳糖摄入可对大鼠血糖、血脂、尿酸代谢产生不同影响,使用时应控制乳糖适宜的摄入剂量。

Objective： To investigate the influence of different concentrations of lactose-sweetened beverage on serum glucose, lipid and uric acid levels in Sprague-Dawley（SD） rats. Methods： Rats were given free access to tap water, 50, 100, 150 g/L lactose-sweetened beverage for eight weeks, respectively. The indexes including food intake, beverage consumption, calories intake and body weight were measured at different time points during the experiment. Serum samples were harvested for determining the levels of blood glucose（GLU）, uric acid（UA）, triglyceride（TG）, total cholesterol（TC）, high-density lipoprotein cholesterol（HDL-C） and low-density lipoprotein cholesterol（LDL-C）. Results： Compared with the control group, the total energy intake reduced signifi cantly; moreover, the body weight in the 1st and 2nd week decreased with statistical difference in the rats provided with 150 g/L lactose（P〈0.05）. The GLU level in the treatment groups was higher than that in the water control group（P〈0.05 or P〈0.01）. The serum uric acid level in 50 and 100 g/L lactose groups decreased signifi cantly（P〈0.05 or P〈0.01）, while the rats given access to 150 g/L lactose-sweetened beverage showed a temporary increase of uric acid level in the 1st week（P〈0.05）. The TG level in 100 g/L lactose group elevated signifi cantly in the 6th week（P〈0.05）. A signifi cant increase in TG level of rats given 150 g/L lactose was detected from the 1st week to the 3rd week（P〈0.05 or P〈0.01）. In addition, the TC level increased signifi cantly in the 2nd, 3rd and 7th week during this experiment（P〈0.05 or P〈0.01）. A signifi cant increase in LDL-C level was observed in the treatment groups（P〈0.05 or P〈0.01）. Conclusion： Diverse influence on serum glucose, lipid and uric acid level can be exerted by different concentrations of lactose-sweetened beverage. The dose of lactose intake should be restricted in the application process.