不同形态硒与VE联用对H_2O_2诱导人脐静脉血管内皮细胞氧化应激损伤的保护作用

Protective Effect of Individual and Combined Selenium and VE on H_2O_2-Induced Oxidative Damage in HUVECs

目的:研究不同形态硒化合物(有机硒:L-硒甲基硒代半胱氨酸;无机硒:亚硒酸钠)单独使用及其与VE联合使用后对H_2O_2诱导人脐静脉血管内皮细胞(human umbilical vein endothelial cells,HUVECs)产生氧化应激损伤的保护作用。方法:采用体外细胞培养方法,将HUVECs随机分为7组:对照组、H_2O_2组、L-硒甲基硒代半胱氨酸(L-Se-methylselenocysteine,L-Se MSC)组、亚硒酸钠(sodium selenite,SS)组、VE组、L-Se MSC+VE联用组、SS+VE联用组。经不同样品干预处理后,采用四甲基偶氮唑蓝(methyl thiazolyl tetrazolium,MTT)法检测HUVECs存活率;检测各组细胞内脂质氧化终产物丙二醛(malondialdehyde,MDA)含量以及超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性的变化。结果:各处理组HUVECs存活率之间没有显著差异;与H_2O_2组比较,0.1 mg/L亚硒酸钠、10 mg/L VE处理能使HUVECs内MDA生成量显著减少,SOD和GSH-Px活性显著升高,并且亚硒酸钠和VE联合使用在细胞水平上具有显著的协同作用,而L-Se MSC组与对照组无显著差异。结论:亚硒酸钠和VE均可保护由H_2O_2诱导引起的HUVECs氧化损伤,提高HUVECs抗氧化能力,并且亚硒酸钠和VE联用具有明显的协同抗氧化作用。

Objective： To study the protective effect of individual and combined selenium and VE on human umbilical vein endothelial cell（HUVECs） with H_2O_2-induced oxidative damage. Methods： HUVECs cultured in vitro were divided into normal control group, model control group and five treatment groups including L-Se-methylselenoeysteine（L-Se MSC） group, sodium selenite（SS） group, VE group, L-Se MSC ＋ VE group, and SS ＋ VE group. The HUVECs were injured by H_2O_2 after they were incubated for 24 h. The effects of all these treatments on cell viability were assayed by MTT. The activity of antioxidant enzymes（SOD and GSH-Px） and the content of MDA were determined. Results： Compared with the model group, treatment with 0.1 mg/L SS, or 10 mg/L VE could increase the viability of HUVECs, reduce the content of MDA, and increase the activity of antioxidant enzymes（SOD and GSH-Px）. The combination of SS and VE exerted a synergistic antioxidant effect at the cellular level, but L-Se MSC did not exhibit a significant difference when compared with the control group. Conclusion： both SS and VE possess protective effect on H_2O_2-induced damage in HUVECs, and can enhance cell antioxidant activity in a synergistic manner.