天冬氨酸激酶代谢调控的研究进展

Review of Research on Metabolic Regulation of Aspartokinase

在微生物发酵合成天冬氨酸族氨基酸的代谢途径中,天冬氨酸激酶(aspartokinase,AK)是限制碳源和氮源流速的限速酶,是决定能否获得高产天冬氨酸族氨基酸的关键之一。本文介绍了AK的重要性、不同来源AK的整体结构、效应结合位点的特点和调控机制研究进展,并对AK代谢调控的发展做出展望,为进一步明确AK代谢调控机制,促进天冬氨酸族氨基酸产业化发展提供参考和依据。

Aspartate kinase（aspartokinase, AK） is a rate-limiting enzyme controlling the carbon and nitrogen flux into the biosynthesis pathways of amino acids of the aspartic acid family in microorganisms. It is a key enzyme to determine the ability to obtain aromatic aspartic amino acids. This article describes recent progress in understanding the importance of aspartatekinase, the general structure of aspartatekinases from different species, features of their binding sites and their regulatory mechanisms. Moreover, future research trends in this area are foreseen. The aim of this review is to provide further understanding of the mechanism of metabolic regulation of aspartatekinase and accordingly promote the industrial development of aspartic acid family amino acids.