独一味胶囊中2种成分的同时测定及在大鼠血浆中的药动学特征

Simultaneous determination of two constituents in Duyiwei Capsules and their pharmacokinetic characters in rat plasma

目的建立超高效液相串联质谱（UPLC-MS/MS）法同时测定独一味胶囊（独一味）中胡麻属苷和类叶升麻苷,并研究其在大鼠血浆中的药代动力学特征。方法蛋白沉淀法处理血浆后,采用Acquity UPLC BEH C（18）色谱柱（50 mm×2.1 mm,1.7μm）分析;流动相为乙腈-0.1%甲酸水,梯度洗脱;体积流量为0.4 m L/min。结果类叶升麻苷的线性范围为1～250 ng/m L（r=0.998 9）,最低定量限为0.2 ng/m L;胡麻属苷的线性范围为0.5～100 ng/m L（r=0.998 6）,最低定量限为0.1 ng/m L;低、中、高质量浓度的日内、日间精密度RSD均〈8.84%,类叶升麻苷的相对回收率分别为（105.33±8.64）%、（101.55±1.22）%和（96.89±5.42）%,而胡麻属苷分别为（103.89±9.18）%、（99.34±6.63）%和（95.88±3.69）%。大鼠静脉注射胡麻属苷和类叶升麻苷后,两者的t（1/2）分别为（1.45±0.28）h和（0.7±0.25）h,AUC（0-t）分别为（128.79±30.52）ng/（h·m L）和（98.39±16.52）ng/（h·m L）。结论该方法简便、快捷,灵敏,适用于这两种成分的药代动力学研究。

AIM To develop an ultra performance liquid chromatography-tandem mass spectrometry（ UPLCMS/MS） method for the simultaneous determination of sesamoside and verbascoside in Duyiwei Capsules（ Lamiophlomis rotata） and to study their pharmacokinetic characters in rat plasma. METHODS After the plasma was treated with protein precipitation method,the analysis was performed on an Acquity UPLC BEH C（18）column（ 50 mm × 2. 1 mm,1. 7 μm）,mobile phase was acetonitrile-0. 1% formic acid aqueous solution with gradient elution,and flow rate was 0. 4 m L/min. RESULTS The linear range of verbascoside was 1- 250 ng/m L（ r =0. 998 9） with the lowest quantitative limit of 0. 2 ng/m L,while the linear range of sesamoside was 0. 5-100 ng/m L（ r = 0. 998 6） with the lowest quantitative limit of 0. 1 ng/m L. The intra-day and inter-day RSDs of low,medium and high concentrations were all less than 8. 84%,the average recoveries of verbascoside were（ 105. 33 ± 8. 64） %,（ 101. 55 ± 1. 22） % and（ 96. 89 ± 5. 42） %,while those of sesamoside were（ 103. 89 ±9. 18） %,（ 99. 34 ± 6. 63） % and（ 95. 88 ± 3. 69） %,respectively. After rats were intravenously injected with sesamoside and verbascoside,their t（1/2）were（ 1. 45 ± 0. 28） h and（ 0. 7 ± 0. 25） h,and AUC（ 0- t）were（ 128. 79 ± 30. 52） ng/（ h·m L） and（ 98. 39 ± 16. 52） ng/（ h·m L）,respectively. CONCLUSION This method is simple,rapid and sensitive,which is suitable for the pharmacokinetic study of these two constituents.