ITP患者血清IgG型抗体糖基化特异性的研究

Study on the glycosylation specificity of the IgG antibody in ITP

目的分析ITP患者血清中IgG型抗体糖基化的特异性,初步探讨其特异性与临床症状的相关性。方法选取健康献血员30例、ITP患者13例及健康怀孕妇女23例,纯化血清中的IgG型抗体,应用nano-LC-MS法分析糖基化种类及水平,对比分析ITP患者血清中IgG型抗体的糖基化特异性。结果 1 ITP患者血清中IgG型抗体的半乳糖糖基化水平为47.08±2.69,低于健康怀孕妇女(50.93±2.21),高于健康献血员(42.88±2.00)(P<0.05);2 ITP患者岩藻糖基化水平为81.16±2.49低于健康献血员(82.60±2.56)(P>0.05),同时低于健康怀孕妇女(86.17±2.23)(P<0.05);3ITP患者唾液酸化水平为3.93±1.20,高于健康献血员(3.69±1.19),低于健康怀孕妇女(4.28±0.88)(P>0.05)。4ITP患者乙酰葡糖氨基化水平为10.53±1.41,低于健康献血员(11.54±1.76),高于健康怀孕妇女(10.13±1.45)(P>0.05)。结论 ITP患者血清中的IgG型抗体的岩藻糖基化、半乳糖糖基化水平的特异性可能是其产生有别于健康怀孕妇女的临床症状的的分子基础。

Objective To investigate the glycosylation specificity of the IgG antibody in ITP. Methods Choose 30 healthy donors,23 healthy pregnants and 13 ITP patients,purified the IgG antibody from serum,analysied the level of all kinds of glycosylation. Compared with healthy donor and healthy pregnants to find the specificity of the IgG antibody in ITP patients.Results 1 The galacosylation of IgG antibody in ITP patients was 47. 08 ± 7. 24,lower than healthy pregnants（ 50. 93 ±4. 91）,higher than healthy donor（ 42. 88 ± 4. 01）,and the healthy pregnants were higher than healthy donor（ P〈0. 05）. 2The fucosylation in ITP patients was 81. 16 ± 6. 2,lower than healthy donors（ 82. 60 ± 2. 56）（ P〉0. 05）,higher than healthy pregnants（ 86. 17 ± 2. 23）（ P〈0. 05）; 3The sialylation in ITP patients was 3. 93 ± 1. 20,higher than healthy donors（ 3. 69± 1. 19）,lower than healthy pregnants（ 4. 28 ± 0. 88）（ P〉0. 05）; 4The Glc NAc in ITP patients was 10. 53 ± 1. 41,lower than healthy donors（ 11. 54 ± 1. 76）,higher than healthy pregnants（ 10. 13 ± 1. 45）（ P〉0. 05）. Conclusion The specificity of IgG antibody in ITP patients in galacosylation and fucosylation may be the molecule base of ITP＇s clinic symptom.