水分胁迫对紫色不结球白菜花色苷合成及相关基因表达的影响

Anthocyanins Biosynthesis and Gene Expression Changes of Non-heading Chinese Cabbage under Water Stress

用250g·L-1的聚乙二醇(PEG 6000)溶液模拟水分胁迫处理‘紫冠1号’紫色不结球白菜,运用液质联用和荧光定量PCR方法,分析水分胁迫下其叶片中花色苷质量分数、成分的变化以及花色苷合成基因BcPAL、BcCHS、BcCHI、BcF3H、BcDFR、BcANS和BcUFGT的表达特性。结果显示:1水分胁迫诱导不结球白菜积累花色苷;2共检测到11种主要的花色苷组分,分为矢车菊素类花色苷和飞燕草素类花色苷2大类,水分胁迫对不同花色苷质量分数的影响作用不同;3水分胁迫条件下,BcPAL、BcCHS和BcDFR基因的表达先升后降,BcF3H和BcUFGT基因在水分胁迫下被持续诱导表达,BcANS和BcCHI基因的表达量在胁迫初期有所下降,随后又逐渐升高。

250g·L^-1 PEG6000solution（polyethylene glycol 6000,PEG）were dipped in root of purple non-heading Chinese cabbage named‘Ziguan No.1＇and liquid chromatography-mass spectrometry as well as fluorescence quantitative PCR method were used to analyze the effects of water stress on anthocyanins content,composition and the expression characteristics of anthocyanins biosynthesis related genes such as BcPAL,BcCHS,BcCHI,BcF3 H,BcDFR,BcANSand BcUFGTin non-heading Chinese cabbage leaves.The results showed that：①the anthocyanins content showed increasing under water stress;②11kinds of main components of anthocyanins were detected and they were divided into two categories,cyanidin and delphinidin,and the influence of water stress on contents of different anthocyanins were different;③the expression of BcPAL,BcCHSand BcDFR under water stress increased first and then decreased,while the expression of BcF3 H and BcUFGT were continuously induced by water stress,BcANSand BcCHIexpression levels declined in the early stress and then gradually increased.