应用SNP标记高效鉴定黄瓜杂交种纯度

Highly Efficient Identification of Seed Purity of Cucumber Hybrid by SNP Marker

以71个市售黄瓜品种为材料,应用高分辨率熔解曲线技术筛选可用于黄瓜纯度检验和品种鉴定的SNP位点,并通过焦磷酸测序验证,得到14个SNP位点,这些位点在71个市售黄瓜品种中的多态信息量为0.079～0.528。为确保结果的准确性,利用该鉴定方法检测黄瓜杂交种F1的94粒种子,结果显示,Chr0108等6个SNP位点检测的种子纯度均为100%,且鉴定结果与SSR标记检测方法相符。表明,对供试样品同时进行这6个SNP位点的检测分析,能够更加可靠、准确、高效地鉴定其纯度,并可用于DUS测试分析,为今后葫芦科作物的品种审定和新品种保护等工作奠定基础。

To test the purity of hybrid of cucumber（Cucumis sativus L.）,14 SNP site was screened by high resolution melting（HRM）and pyrosequencing technology from 71 commercial cucumber cultivars,of which polymorphism information（PIC）were 0.079-0.528.To verify the accuracy of the results in purity test,94F1 hybrid seeds were screened and identified using 6SNP markers.The result showed that the seed purity was 100%,which was consistent with the results identified by SSR method overall,6SNP markers for identification of purity in testing samples can be more reliable,accurate,and efficient method,and can be used in DUS testing,which laid the foundation of cucurbit crops variety examination and protection of new varieties for the future.