高分辨率溶解曲线分析miRNA-196a2基因多态性与肺癌相关性

High resolution melting analysis of the correlation of the miRNA-196a2 gene polymorphism and lung cancer

目的探讨microRNA-196a2基因多态性（rs1614913）与中国人群肺癌发病相关性。方法应用高分辨率溶解曲线（high resolution melting,HRM）分析技术对32例肺癌患者、27例肺部良性肿瘤患者及84例健康个体的microRNA-196a2基因多态性进行分析,并采用聚合酶链反应-限制性片段长度多态性（PCR-restriction fragment length polymorphism,PCRRFLP）技术加以佐证。结果 micro-196a2在各组基因型分布均达到Hardy-Weinberg平衡。肺癌组C等位基因的分布频率高于对照组（P=0.0016）,OR=2.592（95%CI1.424～4.716）,说明C等位基因增加肺癌患病风险。结论 MicroRNA-196a2T/C（rs1614913）多态性增加肺癌的发病风险。MicroRNA-196a2基因多态性rs1614913可能是肺癌的一种有效的遗传标记物。

Objective To investigate the effect of the microRNA-1962 gene polymorphism on risk of lung cancer.MethodsThirty-two patients with lung cancer,27 patients with benign neoplasm in lung and 84 healthy controls.All subjects were genotyped for the microRNA-196a2 SNP by high resolution melting analysis,and the results were supported by PCR-restriction fragment length polymorphism method.Results The microRNA 1962 agenotype distribution among three group was in Hardyweinberg equilibrium.The frequency of the C allele in group of lung cancer was higher than that of the control group（P=0.0016）.The odds ratio 2.592（95% CI 1.424～4.716）suggested an association of the C allele with increased risk of lung cancer.Conclusions The microRNA-196a2T/C polymorphism（rs1614913）is associated with an increased risk of lung cancer.The miRNA-196a2 functional polymorphism rs11614913 might be an effective genetic marker for lung cancer.