羟基红花黄色素A对小鼠肝癌移植瘤NF-κB信号通路的影响

The effect of hydroxysafflor yellow A on NF-κB signaling pathway in transplanted hepatocellula carcinoma in mice

目的研究羟基红花黄色素A(HSYA)对小鼠H22肝癌移植瘤NF-κB信号通路的影响。方法建立H22移植性肝癌小鼠模型50只,随机分为HSYA高剂量组(4.5 mg/kg)、HSYA中剂量组(2.25 mg/kg)、HSYA低剂量组(1.125 mg/kg)、阳性对照组(50mg/kg)和生理盐水对照组,给药14d。免疫印迹检测核转录因子-κB(NF-κB)p65、磷酸化NF-κB抑制蛋白(p-IκB-α)及NF-κB抑制蛋白(IκB-α)的水平。结果 HSYA高、中、低剂量组能够下调细胞核p65蛋白的表达,抑制活化p65核移位,抑制IκB-α的磷酸化和胞质内IκB-α的降解即降低NF-κB的转录活性,尤以中剂量组效果最显著。结论 HSYA能抑制小鼠肝癌移植瘤NF-κB的转录活性。

Objective To investigate the effect of HSYA on NF-κB signaling pathway in H22 transplanted hepatocellula carcinoma in mice.Methods H22-transplanted hepatocelluar carcinoma model in mice was established in this study.fifty mice injected with H22 cells were randomly divided into HSYA high group（4.5mg/kg）,HSYA medium group（2.25mg/kg）,HSYA low group（1.125mg/kg）,positive control group（50mg/kg）and normal saline（NS）group,and were treated for 14 days respectively.Western blotting was performed to analyze the expression of NF-κB p65 in the nucleus,phosphonated inhibitor of nuclear factor kappaB（p-IκB-α）and IκB-αin tumor tissues.Results The results showed that HSYA high,medium and low groups could reduce the expression of p65 in the nuclear fraction,suggesting that HSYA could restrain the translocation of activated p65 into the nucleus.Furthermore,we also found HSYA could inhibit IκB-αphosphorylation and cytoplasmic degradation of IκB-α,leading to a reduced level of NF-κB transactivation.HSYA medium group exerted the most effective function.Conclusions HSYA could inhibit transactivation of NF-κB in transplanted hepatocelluar carcinoma in mice.