维生素E对抗环磷酰胺对肾小管上皮细胞的毒性作用

Vitamin E inhibits the toxic effects of cyclophosphamide on human kidney tubular epithelial cell

目的研究维生素E抑制环磷酰胺所致肾小管上皮细胞毒性作用的影响。方法通过MTT和中性彗星实验,分别观察环磷酰胺单独或与维生素E联合应用时对肾小管上皮细胞生存率及DNA损伤的作用。结果与对照组相比,1、3mol/L环磷酰胺处理肾小管上皮细胞12h后可显著降低细胞生存率,并可造成细胞的DNA损伤(P<0.05);与环磷酰胺处理组相比,1mmol/L维生素E显著降低了环磷酰胺致肾小管上皮细胞DNA损伤的作用(P<0.05),但对环磷酰胺降低肾小管上皮细胞生存率的作用无明显影响(P>0.05)。结论维生素E对抗环磷酰胺致肾小管上皮细胞DNA损伤的作用。

Objective To study the inhibitory action of vitamin E for the toxic effects of cyclophosphamide（CTX）on human kidney tubular epithelial cells（HKCs）.Methods MTT assay and neutral comet assay were separately used to observe the effect of CTX alone and in combine with vitamin E on the survival rate and DNA damage of HKCs.Results After 12 htreatment,1,3mol/LCTX inhibited proliferation of HKCs and induced DNA damage of HKC（P〈0.05）in comparison with control group.Compared with CTX treated group,Vitamin E decreased DNA damage induced by CTX（P〈0.05）.In addition,VE didn’t change the HKC death induced by CTX（P〉0.05）.Conclusions VE could inhibit the DNA damage of HKC induced by CTX.