白藜芦醇对肠缺血再灌注诱发大鼠肺损伤及肺组织肥大细胞活化的影响

Effects of resveratrol on lung injury induced by intestinal ischemia-reperfusion and activation of mast cells in lung tissues of rats

目的探讨白藜芦醇对肠缺血再灌注诱发大鼠肺损伤及肺组织肥大细胞活化的影响。方法清洁级健康雌性SD大鼠30只，体重180～220g，4～5月龄，采用随机数字表法分为3组（n=10）：假手术组（Sham组）、肠缺血再灌注组（II／R组）和白藜芦醇组（Res组）。采用夹闭肠系膜上动脉75min再灌注4h的方法制备大鼠肠缺血再灌注诱发肺损伤模型。Res组于模型建立前5d腹腔注射白藜芦醇15mg／kg，1次，d，连续5d。Sham组与II／R组腹腔注射等容量生理盐水。于再灌注4h时放血处死大鼠，取肺组织，光镜下观察病理学结果，并行Hofbauer评分，测定湿，干重（W／D）比值，采用比色法检测β-已糖苷酶的含量，采用Western blot法检测类胰蛋白酶的表达。结果与Sham组比较，II／R组和Res组肺组织Hofbauer评分、W／D比值和β-已糖苷酶含量升高，类胰蛋白酶表达上调（P〈0．05）；与II／R组比较，Res组肺组织Hofbauer评分、W／D比值和β-已糖苷酶含量降低，类胰蛋白酶表达下调（P〈0．05）。Res组肺组织病理学改变较II／R组减轻。结论白藜芦醇可减轻肠缺血再灌注诱发大鼠肺损伤，其机制可能与抑制肺组织肥大细胞活化有关。

Objective To investigate the effects of resveratrol on lung injury induced by intestinal ischemia-reperfusion （I/R） and activation of mast cells in lung tissues of rats. Methods Thirty healthy female Sprague-Dawley rats, weighing 180-220 g, aged 4-5 months, were randomly divided into 3 groups （n= 10 each） using a random number table： sham operation group （Sham group）, intestinal I/R group （II/R group） , and resveratrol group （Res group）. Intestinal I/R was produced by occlusion of the superior mesenteric artery for 75 min followed by 4 h of reperfusion in anesthetized rats. In Res group, resveratrol 15 mg/kg was injected intraperitoneally once a day for 5 consecutive days before I/R. The equal volume of normal saline was given in Sham and II/R groups. All the rats were sacrificed at 4 h of reperfusion, and the lungs were removed for examination of the pathological changes which were scored and for determination of wet/dry lung weight ratio （W/D ratio）, content of [3-hexosaminidase （by colorimetric method）, and expression of tryptase in lungs tissues （ by Western blot）. Results Compared with Sham group, the pathological score, W/D ratio, and 13-hexosaminidase content were significantly increased, and the expression of tryptase was up-regulated in II/R and Res groups （P〈0.05）. Compared with II/R group, the pathological score, W/D ratio, and 13-hexosaminidase content were significantly decreased, and the expression of tryptase was down-regulated in Res group （P 〈 0.05 ）. The pathological changes were significantly reduced in Res group as compared with II/R group. Conclusion Resveratrol can attenuate lung injury induced by intestinal I/R, and the mechanism may be related to inhibition of the activation of mast cells in lung tissues of rats.