miR-496对肝癌细胞株HepG2细胞周期的影响

Effect of miR-496 on cycle of HepG2 cells

为探究miR-496对肝癌细胞株HepG2细胞周期的影响,通过Lip2000分别转染miR-496模拟物、模拟物的阴性对照、miR-496抑制物、抑制物阴性对照于肝癌细胞株HepG2细胞中,经PCR验证转染成功后,应用流式细胞术检测miR-496对肝癌细胞株HepG2细胞周期的影响.结果显示:加入miR-496模拟物的HepG2细胞检测结果与对照组相比,G0/G1期细胞数上升了10%,G2/M期细胞数下降了7%;加入miR-496模拟物阴性对照物的检测结果与对照组结果基本没变化;而加入miR-496抑制物的检测结果与对照组结果相比,G0/G1期细胞数减少,G2/M期细胞数增加;加入miR-496抑制物阴性对照组的检测结果相对于对照组变化不显著.结果表明:miR-496可以抑制细胞HepG2的分裂.

To study the effect of miR-496 on cycle of HepG2 cells,the experiment use Lip 2000 transfected miR-496 mimics,mimetic negative control,inhibitor,inhibitor negative control in hepatoma cell line HepG2. Successful transfection confirmed by PCR,the effect of miR-496 on cycle of HepG2 cells was detected by Nucleocounter（ R） NC-3000 TM. In the join miR-496 mimics HepG2 test,compared with the control group,G0 / G1 phase cells increased,G2 / M phase cells decreased. Join miR-496 mimics negative control test results with the control group did not change the basic results. Join miR-496 inhibitor test results with the control group compared to the results,G0 / G1 phase cells decreased,and G2 / M phase cells increased. In the case of the miR-496 inhibitor added to the negative control group,the detection result is basically unchanged. In summary,miR-496 can inhibit the cell line HepG2 division.