肥肝鹅恢复期血液生化指标、肝脏常规营养成分及脂代谢相关基因表达水平的变化研究

Study on Changes of Blood Chemistry Indexes,Hepatic Routine Nutritional Composition and Expression of Lipid Metabolism-associated Genes during the Recovery of Geese with Fatty Livers

试验旨在研究朗德鹅填饲形成肥肝及恢复后其体重、肝脏重、腹脂重、常规营养成分、血液生化指标和肝脏中与脂代谢相关基因的表达变化情况,为进一步阐明鹅肥肝的恢复或保护机制提供依据。将18只70日龄朗德鹅随机分为3组(每组6只):组1为对照组,用玉米饲料进行常规饲喂;组2为填饲组,填饲玉米饲料19d;组3为填饲19d后再用玉米饲料常规饲养20d进行肝脏恢复。结果显示,与组2相比,组3鹅体重、肝脏重均极显著降低(P<0.01),而腹脂重下降不显著(P>0.05);与组2相比,组3肝脏水分、灰分和粗蛋白质均显著上升(P<0.05),而粗脂肪含量显著下降(P<0.05),但与组1相比,除水分外,各项指标均无显著变化(P>0.05);与组2相比,组3血浆中谷丙转氨酶(ALT)、谷草转氨酶(AST)、甘油三酯(TG)和高密度脂蛋白胆固醇(HDL-C)浓度与组2相比均显著下降(P<0.05),但与组1相比,均无显著差异(P>0.05);与组2相比,组3肝脏中脂肪酸脱氢酶(FADS1)、硬脂酰辅酶A去饱和酶(SCD1)和细胞色素P450 2C45(CYP2C45)基因的表达量均显著下降(P<0.05),但与组1相比均无显著变化(P>0.05)。综上所述,本研究从不同层面揭示了鹅肥肝的可逆性,为深入研究鹅肥肝的恢复或保护机制,促进动物脂肪肝问题的解决奠定了基础。

This experiment aimed to determine the changes of body weight,liver weight,abdominal fat weight,hepatic routine nutritional composition,blood biochemistry indexes,as well as mRNA expression of lipid metabolism-associated genes during the recovery of Landes geese with fatty liver,which provided the basis for the further elucidation of the recovery or protection mechanism of goose fat liver.18 Landes geese were divided into three groups（6gees in each group）.Group 1was control group,in which the geese were fed with boiled maize;Group 2was the overfeeding group without recovery,in which the geese were overfed with a maize-based diet for 19days;And group 3was the overfeeding group with 20-day recovery,in which the geese were overfed with the maize-based diet for 19 days followed by feeding boiled maize for 20-day recovery.The results showed that compared with group 2,the body weight and liver weight of the geese in group 3decreased extremely significantly（P〈0.01）,and the abdominal fat weights undecreased signifi-cantly（P〉0.05）;Moreover,the percentages of nutrients including water,ash,CP of livers in group 3increased significantly（P〈0.05）,while the percentage of EE decreased significantly（P〈0.05）,but there were no significant difference for these variables between groups 1and 3（P〉0.05）except water.In regard to blood biochemistry indexes,the contents of blood ALT,AST,TG and HDL-C in group 3decreased significantly（P〈0.05）,but there were no significant difference from those of group 1（P〉0.05）.The mRNA expression levels of FADS1,SCD1 and CYP2C45in group 3decreased significantly compared with group 1（P〈0.05）,but there were no significant difference between groups 1and 3（P〉0.05）.In summary,this study demonstrated the recoverability of goose fatty liver at different layers,and thus laid a foundation for further investigation on the recovery or protective mechanism of goose fatty liver and found the solution to resolve the problems related to animal fatty liver.