断奶仔猪对不同锰源生物学利用率的研究

The Relative Bioavailability of Mn Sources in Weaned Piglets

本试验旨在研究断奶仔猪对甘氨酸锰和复合氨基酸锰(有机锰源)相对无机硫酸锰的生物学利用率。采用3×2两因子完全随机设计,将224头体况良好、体重相近(9.67±0.13)kg三元杂交(杜×长×大)断奶仔猪分成7个处理组,每个处理4个重复。对照组饲喂不额外添加锰的基础日粮(锰含量为39.22mg/kg),试验组在对照组的基础上分别以无机硫酸锰、甘氨酸锰和复合氨基酸锰的形式添加20、40mg/kg。预饲期7d,正饲期28d。结果表明,锰源及锰源与锰水平的互作对断奶仔猪的生产性能、血清锰含量、锰超氧化物歧化酶(Mn-SOD)活性及肾脏、心脏、胰脏锰含量均无显著影响(P>0.05),但肝脏和跖骨锰含量受锰源及锰水平的影响显著(P<0.05),根据肝脏锰和跖骨锰含量与日粮锰进食量之间拟合的多元线性回归,运用斜率比率法,计算甘氨酸锰和复合氨基酸锰相对无机硫酸锰(100%)的生物学利用率分别是125.95%,112.79%和133.08%,119.25%,表明断奶仔猪对甘氨酸锰和复合氨基酸锰的生物学利用率显著高于无机硫酸锰(P<0.05),但有机锰源间无显著差异(P>0.05)。

The objective of this study was to determine the bioavailability of organic Mn（Mn-Gly and Mn-AA）relative to inorganic source（MnSO4· H2O）in weaned piglets.A total of two hundreds and twenty-four post-weaning piglets（Duroc×Landrace×Yorkshire）with（9.67±0.13）kg body weight were randomly allotted to seven treatments（eight piglets per replicate）.3×2two factors completely random design was used with two levels of added Mn（20,40mg/kg）and three Mn sources（Mn-Gly,Mn-AA and Mn sulfate）,and a control group with no added Mn was included.The experimental phase was 35 days,including 7days adaption.The results showed that the growth performance,Mn-SOD activity,Mn content in serum,heart,kidney and pancreas were not significantly affected by Mn sources or Mn sources×Mn levels interaction（P〉0.05）.However,the liver and bone Mn contents were significantly affected by Mn source and Mn level（P〈0.05）.Based on slope ratios from multiple linear regression between Mn contents in liver and metatarsal bone and dietary supplemental Mn intake,the relative bioavailabilities of Mn-Gly and Mn-AA were 125.95% and 112.79% for liver Mn content and 133.08% and 119.25% for metatarsal bone Mn content compared to 100%of Mn sulfate,respectively,indicating that the bioavailabilities of Mn-Gly and Mn-AA were significantly higher than Mn sulfate（P〈0.05）,but there were no significant differences between Mn-Gly and Mn-AA（P〈0.10）.